Visualisering af bakteriofager: Visualization of bacteriophages

Frederik H. Aidt, Christian M. Hagen, Jens E. Olsen & Christian T.F. Nielsen

Student thesis: Termpaper

Abstract

Bakterien Lactococcus lactis subsp. lactis IL1403 blev inficeret med bakteriofagen bIL170, farvet med de fluorescerende farvestoffer SYBR Gold og DAPI med henblik på at følge infektionen i fluorescensmikroskop. Det blev vist at fag bIL170 ikke blev inaktiveret ved farvning med SYBR Gold eller DAPI inden for 48 timer. Det blev yderligere vist at reprodutionscyklus for fag bIL170 forlænges med ca. 25% ved farvning med SYBR Gold. Ingen forsinkelse blev fundet ved farvning med DAPI. Det konkluderedes at SYBR Gold er velegnet til farvning af fag bIL170. Det har ikke været muligt at følge infektionsprocessen af SYBR Gold farvede fager i fluorescensmikroskop på et almindeligt objektglas. Lysis af bakterier inficerede med hhv. SYBR Gold farvede fager og ufarvede fager, udtaget fra flydende kultur er dog blevet observeret under mikroskop. The bacteria Lactococcus lactis subsp. lactis IL1403 was infected with bacteriophage bIL170, stained with the fluorescent dyes SYBR Gold and DAPI with the purpose of observing the infection in fluorescence microscope. It was shown that phage bIL170 was not inactivated due to staining with either SYBR Gold or DAPI within 48 hours. Furthermore it was shown that the infection cycle of phage bIL170 is prolonged approximately 25% due to staining with SYBR Gold within 48 hours. No prolongation was found due to staining over night with DAPI. It was concluded that SYBR Gold is a suitable dye for staining of phage bIL170. It has not been possible to follow the infection process of phages stained with SYBR Gold in the fluorescence microscope on an ordinary cover glass. However, lysis of bacteria infected with respectively with SYBR Gold stained phages and unstained phages has been observed under microscope.

EducationsBasic - Bachelor Study Program in Natural Science, (Bachelor Programme) Basic
LanguageDanish
Publication date1 Jun 2005
SupervisorsJens Spanget

Keywords

  • fluorescens
  • bakteriofager
  • bil170
  • SYBR
  • fager
  • bacteriophages
  • phages
  • DAPI
  • lactococcus