Previous experiments have shown a SNP-mutation on the KdgR-gene in Salmonella Typhimurium to be far more effective in the colonization of the intestines, compared to the wild type. In this project, the KdgR-SNP-mutation is examined further. Along side it is examined which effect the virulence genes SPI-1 and -2 have on the colonization, and the effect of the Nramp1-gene of the host. For this project, five groups of mice were used; Group 1 was a control group with the genotype Nramp1-/- and was infected with sterile saline. Group 2 was of the genotype Nramp1-/- and was infected with S. Typhimurium wt and kdgR-SNP. Group 3 was of the genotype Nramp1-/- and was infected with S. Typhimurium wt-ΔSPI og kgdR-SNP-ΔSPI. Group 4 was of the genotype Nramp1+/+ and was infected with S. Typhimurium wt and kgdR-SNP. Group 5 was of the genotype Nramp1+/+ and was infected with S. Typhimurium wt-ΔSPI og kgdR-SNP-ΔSPI. The results of the project shows that S. Typhimurium with inactive SPI-genes is not able to colonize the intestines. It has not been possible to obtain results that indicate that the Nramp1 gene has an inhibitory effect on Salmonella infection, as it earlier has been proven. Unfortunately the results of this project have not been able to confirm, that KdgR-SNP-mutation is more efficient in colonization of the mouse intestine than wild type is. Further testing is required before is can be confirmed that there is no difference between the wild type and the SNP-strain.
|Educations||Pharmaceutical Biology, (Bachelor/Graduate Programme) Undergraduate or graduate|
|Publication date||26 Jun 2016|