There is a state of life known as cryptobiosis, where no metabolic activity exists, in which some living organisms can stay for decades. The tardigrade Richtersius coronifer is capable of entering a compact morphological formation called a tun, a cryptobiotic life stage which protects it from stressful conditi-ons, such as heat shock, desiccation, radioactivity and so on. This report will describe cryptobiosis, heat shock proteins, the taxonomy and biology of Richtersius coronifer, and the theory behind Western blotting. Finally, the report will end up with a scientific paper, where it is concluded whether or not a small heat shock protein called p26, exists in Richtersius coronifer through different life stages. The brine shrimp Artemia salina, in which p26 has already been located, serve as the positive control in the experimental part. Through electrophoresis and Western blotting, the presence of p26 in Richtersius coronifer will be attempted elucidated.
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