In yeast, the synthesis of dNTPs progresses along two pathways, called the de novo pathway and the salvage pathway. Unpublished data from Claus Desler and Lene J. Rasmussen have shown that HeLa cells lacking mitochondrial DNA (ρ0 cells) exhibit a significant lower pool of dNTPs than wild type cells. Assuming that Saccharomyces cerevisiae ρ0 cells also exhibit lowered dNTP pools, we have studied the expression of selected genes involved in nucleotide metabolism. As metabolic pathways are very similar in all eukaryotes, it is likely that the de novo and salvage pathways are conserved between yeast and HeLa cells, thus enabling us to draw parallels between expressions of genes in these cell lines. The main focus of this thesis is on the genes AAH1, ADE13, YNK1, BAS1, CDC21, URK1 and FCY2, due to their involvement in the nucleotide metabolism in S. cerevisiae, in different steps of the de novo and salvage pathway. The selection of genes was based on data provided in a genome wide gene array analysis of gene expression in wild type and ρ0 cells of S. cerevisiae. This data has kindly been made available to us by Lene J Rasmussen. We have studied the selected genes in order to determine if their differential expression in S. cereviciae ρ0 cells, compared to wild type cells, can explain a decrease in dNTP pools. We propose that the decreased dNTP pools observed in ρ0 cells can, at least in part, be explained by differential expression of the tree genes CDC21, AAH1 and ADE13, which are directly involved in the de novo and salvage pathways of nucleotide biosynthesis, as well as BAS1 which encodes for the general transcription factor Bas1p.
|Educations||Molecular Biology, (Bachelor/Graduate Programme) Bachelor|
|Publication date||1 Jan 2004|
- Rho0 celler
- Nucleotide metabolism