Diabetes and wound healing

Rikke Svendsen, Gloire Irakunda, Knudsen List, Karoline Cecilie, Marie Sønderstup-Jensen, Hölmich Rosca & Mette Maria

Student thesis: Termpaper

Abstract

Diabetes is a disease where the glucose level in the blood is high, due to either insulin resistance, impaired insulin sensitivity or no insulin production. The high glucose level causes several complications, one of them being an impaired wound healing process, which might lead to chronic wounds, ulcers. Several factors play a role in the development of ulcers, and recent research indicates that microRNA might play a significant role in skin development and wound healing. The purpose of this project is to identify, which microRNAs are increased or decreased in wound healing among diabetic mice and which processes they effect in the wound healing process. This project was based on a literature review and an experiment. Skin samples from diabetic and non-diabetic mice were used in the experiment. The experiment was divided into three steps. First homogenization was used to release the RNA from the mice skin samples. Then RNA purification was performed to purify and isolate the RNA from the samples. Afterwards microarray was used to synthesize complementary DNA from a RNA template and detect the Ct values of each microRNA in the skin samples of the mice. Ten microRNAs were selected, on the basis of the results of the experiment, for further analysis in regards to wound healing. Furthermore six microRNAs were selected on the basis of present studies, which showed these microRNAs’ influence on wound healing. It was concluded that miR-126-3p, miR-133a, miR-191, miR-193b, miR-320, miR-342-3p, miR-574-3p and miR-150 possibly can influence the impaired wound healing among diabetic mice. The same was concluded for miR-210, miR-203, miR-205 and miR-92a. miR-155 and miR-200b however appeared to stimulate wound healing among diabetic mice.

EducationsBasic - Bachelor Study Program in Natural Science, (Bachelor Programme) Basic
LanguageEnglish
Publication date23 Jun 2014
SupervisorsAnja Elaine Sørensen

Keywords

  • Wound healing
  • microRNA
  • Diabetes