YGFP: a spectral variant of GFP protein

Flemming G. Hansen, Tove Atlung

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    Abstract

    We describe YGFP, a slow bleaching green fluorescent protein (GFP) with unique spectral properties. YGFP is derived from an Escherichia coli codon-optimized synthetic gfp mutant 2 derivative. In addition to the GFP-mut 2 changes, it also carries S202F and T203I substitutions. YGFP can be used as a substitute for yellow fluorescent protein (YFP) in experiments in which two or more fluorescent proteins are fused to different cellular protein components, expanding the ability to study multiple labeled proteins in a cell at once.
    Translated title of the contributionYGFP: en spektral variant af GFP
    Original languageEnglish
    JournalBioTechniques
    Volume50
    Issue number1
    Pages (from-to)411-412
    ISSN0736-6205
    Publication statusPublished - 2011

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