YGFP: a spectral variant of GFP protein

Flemming G. Hansen, Tove Atlung

    Research output: Contribution to journalJournal articleResearchpeer-review


    We describe YGFP, a slow bleaching green fluorescent protein (GFP) with unique spectral properties. YGFP is derived from an Escherichia coli codon-optimized synthetic gfp mutant 2 derivative. In addition to the GFP-mut 2 changes, it also carries S202F and T203I substitutions. YGFP can be used as a substitute for yellow fluorescent protein (YFP) in experiments in which two or more fluorescent proteins are fused to different cellular protein components, expanding the ability to study multiple labeled proteins in a cell at once.
    Translated title of the contributionYGFP: en spektral variant af GFP
    Original languageEnglish
    Issue number1
    Pages (from-to)411-412
    Publication statusPublished - 2011

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