The Role of Product Inhibition as a Yield-Determining Factor in Enzymatic High-Solid Hydrolysis of Pretreated Corn Stover

Søren Nymand Olsen, Kim Borch, Nicolaj Cruys-Bagger, Peter Westh

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Industrially, enzymatic hydrolysis of lignocellulose at high solid content is preferable over low solids due to a reduction in processing costs. Unfortunately, the economic benefits are counteracted by a linear decrease in yield with solid content, referred to as the “solid effect” in the literature. In the current study, we investigate the contribution of product inhibition to the solid effect (7–33 % solids). Product inhibition was measured directly by adding glucose to high-solid hydrolysis samples and indirectly through variation of water content and beta-glucosidase concentration. The results suggest that the solid effect is mainly controlled by product inhibition under the given experimental conditions (washed pretreated corn stover as substrate). Cellobiose was found to be approximately 15 times more inhibitory than glucose on a molar scale. However, considering that glucose concentrations are at least 100 times higher than cellobiose concentrations under industrial conditions, glucose inhibition of cellulases is suggested to be the main cause of the solid effect.
    Industrially, enzymatic hydrolysis of lignocellulose at high solid content is preferable over low solids due to a reduction in processing costs. Unfortunately, the economic benefits are counteracted by a linear decrease in yield with solid content, referred to as the “solid effect” in the literature. In the current study, we investigate the contribution of product inhibition to the solid effect (7–33 % solids). Product inhibition was measured directly by adding glucose to high-solid hydrolysis samples and indirectly through variation of water content and beta-glucosidase concentration. The results suggest that the solid effect is mainly controlled by product inhibition under the given experimental conditions (washed pretreated corn stover as substrate). Cellobiose was found to be approximately 15 times more inhibitory than glucose on a molar scale. However, considering that glucose concentrations are at least 100 times higher than cellobiose concentrations under industrial conditions, glucose inhibition of cellulases is suggested to be the main cause of the solid effect.
    LanguageEnglish
    JournalApplied Biochemistry and Biotechnology
    Volume174
    Issue number1
    Pages146-155
    ISSN0273-2289
    DOIs
    StatePublished - 16 Jul 2014

    Keywords

    • High-solid hydrolysis
    • Solid effect
    • Cellulase inhibition
    • Pretreated corn stover

    Cite this

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    title = "The Role of Product Inhibition as a Yield-Determining Factor in Enzymatic High-Solid Hydrolysis of Pretreated Corn Stover",
    abstract = "Industrially, enzymatic hydrolysis of lignocellulose at high solid content is preferable over low solids due to a reduction in processing costs. Unfortunately, the economic benefits are counteracted by a linear decrease in yield with solid content, referred to as the “solid effect” in the literature. In the current study, we investigate the contribution of product inhibition to the solid effect (7–33 {\%} solids). Product inhibition was measured directly by adding glucose to high-solid hydrolysis samples and indirectly through variation of water content and beta-glucosidase concentration. The results suggest that the solid effect is mainly controlled by product inhibition under the given experimental conditions (washed pretreated corn stover as substrate). Cellobiose was found to be approximately 15 times more inhibitory than glucose on a molar scale. However, considering that glucose concentrations are at least 100 times higher than cellobiose concentrations under industrial conditions, glucose inhibition of cellulases is suggested to be the main cause of the solid effect.",
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    doi = "10.1007/s12010-014-1049-1",
    language = "English",
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    The Role of Product Inhibition as a Yield-Determining Factor in Enzymatic High-Solid Hydrolysis of Pretreated Corn Stover. / Nymand Olsen, Søren; Borch, Kim; Cruys-Bagger, Nicolaj; Westh, Peter.

    In: Applied Biochemistry and Biotechnology, Vol. 174, No. 1, 16.07.2014, p. 146-155.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - The Role of Product Inhibition as a Yield-Determining Factor in Enzymatic High-Solid Hydrolysis of Pretreated Corn Stover

    AU - Nymand Olsen,Søren

    AU - Borch,Kim

    AU - Cruys-Bagger,Nicolaj

    AU - Westh,Peter

    PY - 2014/7/16

    Y1 - 2014/7/16

    N2 - Industrially, enzymatic hydrolysis of lignocellulose at high solid content is preferable over low solids due to a reduction in processing costs. Unfortunately, the economic benefits are counteracted by a linear decrease in yield with solid content, referred to as the “solid effect” in the literature. In the current study, we investigate the contribution of product inhibition to the solid effect (7–33 % solids). Product inhibition was measured directly by adding glucose to high-solid hydrolysis samples and indirectly through variation of water content and beta-glucosidase concentration. The results suggest that the solid effect is mainly controlled by product inhibition under the given experimental conditions (washed pretreated corn stover as substrate). Cellobiose was found to be approximately 15 times more inhibitory than glucose on a molar scale. However, considering that glucose concentrations are at least 100 times higher than cellobiose concentrations under industrial conditions, glucose inhibition of cellulases is suggested to be the main cause of the solid effect.

    AB - Industrially, enzymatic hydrolysis of lignocellulose at high solid content is preferable over low solids due to a reduction in processing costs. Unfortunately, the economic benefits are counteracted by a linear decrease in yield with solid content, referred to as the “solid effect” in the literature. In the current study, we investigate the contribution of product inhibition to the solid effect (7–33 % solids). Product inhibition was measured directly by adding glucose to high-solid hydrolysis samples and indirectly through variation of water content and beta-glucosidase concentration. The results suggest that the solid effect is mainly controlled by product inhibition under the given experimental conditions (washed pretreated corn stover as substrate). Cellobiose was found to be approximately 15 times more inhibitory than glucose on a molar scale. However, considering that glucose concentrations are at least 100 times higher than cellobiose concentrations under industrial conditions, glucose inhibition of cellulases is suggested to be the main cause of the solid effect.

    KW - High-solid hydrolysis

    KW - Solid effect

    KW - Cellulase inhibition

    KW - Pretreated corn stover

    KW - High-solid hydrolysis

    KW - Solid effect

    KW - Cellulase inhibition,

    KW - Pretreated corn stover

    U2 - 10.1007/s12010-014-1049-1

    DO - 10.1007/s12010-014-1049-1

    M3 - Journal article

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    SP - 146

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    JO - Applied Biochemistry and Biotechnology

    T2 - Applied Biochemistry and Biotechnology

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    IS - 1

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