The Mobilome: Metagenomic Analysis of Circular Plasmids, Viruses, and Other Extrachromosomal Elements

Patrick Denis Browne, Witold Kot, Tue Sparholt Jørgensen, Lars Hestbjerg Hansen*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Isolation, sequencing, and analysis of circular genetic elements bring new insights to mobile genetic elements related to microbial ecology. One method used to study circular plasmids, viruses, and other elements is called the mobilome method. The mobilome method presented here is an unamplified mobilome approach allowing fast isolation of circular DNA elements from a variety of samples followed by directly building unamplified Illumina-compatible sequencing libraries using enzymatic tagging and fragmentation. Several methods for bioinformatic analysis of mobilome data are also suggested.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Number of pages12
PublisherHumana Press
Publication date2020
Pages253-264
ISBN (Print)978-1-4939-9876-0
ISBN (Electronic)978-1-4939-9876-0
DOIs
Publication statusPublished - 2020
SeriesMethods in Molecular Biology
Volume2075
ISSN1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2020.

Keywords

  • Circular DNA elements
  • Horizontal gene transfer
  • Metagenomics
  • MGE
  • Mobile genetic elements
  • Mobilome
  • Plasmid
  • Plasmidome

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