Isolation, sequencing, and analysis of circular genetic elements bring new insights to mobile genetic elements related to microbial ecology. One method used to study circular plasmids, viruses, and other elements is called the mobilome method. The mobilome method presented here is an unamplified mobilome approach allowing fast isolation of circular DNA elements from a variety of samples followed by directly building unamplified Illumina-compatible sequencing libraries using enzymatic tagging and fragmentation. Several methods for bioinformatic analysis of mobilome data are also suggested.
|Title of host publication||Methods in Molecular Biology|
|Number of pages||12|
|Publication status||Published - 2020|
|Series||Methods in Molecular Biology|
Bibliographical notePublisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2020.
- Circular DNA elements
- Horizontal gene transfer
- Mobile genetic elements