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The microalga Rhodomonas salina is widely used in aquaculture. There is a need for optimization of the growth of the microalgae and its content of essential fatty acids. Here, the fatty acid profile of Rhodomonas in relation to cell density during cultivation in a tubular PBR is investigated. It is expected that cell density is an important factor in controlling productivity and fatty acid content of the microalgae because cell density is important in determining light availability due to the self‐shading of the algae. The carbon productivity as a function of cell density is described by a saturation curve. The carbon productivity and the productivity of total fatty acids are lowest at the lowest cell density, and independent of cell density at higher cell densities. The relative contribution of the two poly‐unsaturated fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) increases with increasing cell density and saturates at 1 × 106 cells/ml. We conclude that large‐scale production of Rhodomonas in this tubular PBR should take place at cell densities of 1 × 106 cells/ml, while there are indications for increasing difficulties in maintaining steady‐state production in this PBR at higher cell densities.