The effect of cell density on biomass and fatty acid productivity during cultivation of Rhodomonas salina in a tubular photobioreactor

Christina Thoisen, Jakob Skov Pedersen, Lars Jørgensen, Anker Kyhn, Benni Winding Hansen, Søren Laurentius Nielsen*

*Corresponding author

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The microalga Rhodomonas salina is widely used in aquaculture. There is a need for optimization of the growth of the microalgae and its content of essential fatty acids. Here, the fatty acid profile of Rhodomonas in relation to cell density during cultivation in a tubular PBR is investigated. It is expected that cell density is an important factor in controlling productivity and fatty acid content of the microalgae because cell density is important in determining light availability due to the self‐shading of the algae. The carbon productivity as a function of cell density is described by a saturation curve. The carbon productivity and the productivity of total fatty acids are lowest at the lowest cell density, and independent of cell density at higher cell densities. The relative contribution of the two poly‐unsaturated fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) increases with increasing cell density and saturates at 1 × 106 cells/ml. We conclude that large‐scale production of Rhodomonas in this tubular PBR should take place at cell densities of 1 × 106 cells/ml, while there are indications for increasing difficulties in maintaining steady‐state production in this PBR at higher cell densities.
Original languageEnglish
JournalAquaculture Research
Volume51
Issue number8
Pages (from-to)3367-3375
Number of pages9
ISSN1355-557X
DOIs
Publication statusPublished - 12 Jul 2020

Bibliographical note

Important note from the Publisher regarding the attached version of the article: "This is the peer reviewed version of the following article: Thoisen, C, Pedersen, JS, Jørgensen, L, Kuehn, A, Hansen, BW, Nielsen, SL. The effect of cell density on biomass and fatty acid productivity during cultivation of Rhodomonas salina in a tubular photobioreactor. Aquac Res. 2020; 51: 3367– 3375, which has been published in final form at https://doi.org/10.1111/are.14672. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."

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