Four groups with three pigs in each group were inoculated with Oesophagostomum dentatum larvae (L3 larvae). Groups 1 and 3 were inoculated with 20 000 larvae, and Groups 2 and 4 with 200 000 larvae. On Days 11 and 34, respectively, Groups 1 and 2 and Groups 3 and 4 were slaughtered, and the contents from the large intestines collected. Subsamples of intestinal contents were mixed with agar to a final concentration of 1% agar and allowed to set. The worms were allowed to migrate from the agar-gel into 38°C 0.9% saline overnight. Then the worms were collected on a sieve (38 μm mesh) and counted. The worms retained in the agar-gel were counted after pouring the melted agar through a sieve (38 μm mesh). The results showed that more than 95% of the worms migrated out of the agar-gel, and subsequently were available for counting in an almost clean suspension. Additionally the method yielded a high worm recovery; all stages were recovered. The recovery percentage was not significantly affected by either the dose of parasites or the time interval from slaughtering to start of incubation (37-128 min).
Bibliographical noteFunding Information:
This study was supported by the Danish National Research Foundation. The skilful technical assistance of enthusiastic technicians, scientists and animal attendants is gratefully acknowledged.
- Agar-gel technique
- Oesophagostomum dentatum
- Worm recovery