Reconciliation of opposing views on membrane-sugar interactions

Heidi Delcomyn Andersen, Chunhua Wang, Lise Arleth, Günther H.J. Peters, Peter Westh

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

It is well established that small sugars exert different types of stabilization of biomembranes both in vivo and in vitro. However, the essential question of whether sugars are bound to or expelled from membrane surfaces, i.e., the sign and size of the free energy of the interaction, remains unresolved, and this prevents a molecular understanding of the stabilizing mechanism. We have used small-angle neutron scattering and thermodynamic measurements to show that sugars may be either bound or expelled depending on the concentration of sugar. At low concentration, small sugars bind quite strongly to a lipid bilayer, and the accumulation of sugar at the interface makes the membrane thinner and laterally expanded. Above ∼0.2 M the sugars gradually become expelled from the membrane surface, and this repulsive mode of interaction counteracts membrane thinning. The dual nature of sugar–membrane interactions offers a reconciliation of conflicting views in earlier reports on sugar-induced modulations of membrane properties.

Original languageEnglish
JournalProceedings of the National Academy of Science of the United States of America
Volume108
Issue number5
Pages (from-to)1874-1878
ISSN0027-8424
DOIs
Publication statusPublished - 2011

Keywords

  • membrane interface
  • membrane structure
  • preferential binding
  • preferential exclusion
  • interaction free energy

Cite this

Delcomyn Andersen, Heidi ; Wang, Chunhua ; Arleth, Lise ; Peters, Günther H.J. ; Westh, Peter. / Reconciliation of opposing views on membrane-sugar interactions. In: Proceedings of the National Academy of Science of the United States of America. 2011 ; Vol. 108, No. 5. pp. 1874-1878.
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Reconciliation of opposing views on membrane-sugar interactions. / Delcomyn Andersen, Heidi ; Wang, Chunhua; Arleth, Lise; Peters, Günther H.J.; Westh, Peter.

In: Proceedings of the National Academy of Science of the United States of America, Vol. 108, No. 5, 2011, p. 1874-1878.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Reconciliation of opposing views on membrane-sugar interactions

AU - Delcomyn Andersen, Heidi

AU - Wang, Chunhua

AU - Arleth, Lise

AU - Peters, Günther H.J.

AU - Westh, Peter

PY - 2011

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N2 - It is well established that small sugars exert different types of stabilization of biomembranes both in vivo and in vitro. However, the essential question of whether sugars are bound to or expelled from membrane surfaces, i.e., the sign and size of the free energy of the interaction, remains unresolved, and this prevents a molecular understanding of the stabilizing mechanism. We have used small-angle neutron scattering and thermodynamic measurements to show that sugars may be either bound or expelled depending on the concentration of sugar. At low concentration, small sugars bind quite strongly to a lipid bilayer, and the accumulation of sugar at the interface makes the membrane thinner and laterally expanded. Above ∼0.2 M the sugars gradually become expelled from the membrane surface, and this repulsive mode of interaction counteracts membrane thinning. The dual nature of sugar–membrane interactions offers a reconciliation of conflicting views in earlier reports on sugar-induced modulations of membrane properties.

AB - It is well established that small sugars exert different types of stabilization of biomembranes both in vivo and in vitro. However, the essential question of whether sugars are bound to or expelled from membrane surfaces, i.e., the sign and size of the free energy of the interaction, remains unresolved, and this prevents a molecular understanding of the stabilizing mechanism. We have used small-angle neutron scattering and thermodynamic measurements to show that sugars may be either bound or expelled depending on the concentration of sugar. At low concentration, small sugars bind quite strongly to a lipid bilayer, and the accumulation of sugar at the interface makes the membrane thinner and laterally expanded. Above ∼0.2 M the sugars gradually become expelled from the membrane surface, and this repulsive mode of interaction counteracts membrane thinning. The dual nature of sugar–membrane interactions offers a reconciliation of conflicting views in earlier reports on sugar-induced modulations of membrane properties.

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KW - membrane structure

KW - preferential binding

KW - preferential exclusion

KW - interaction free energy

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JF - Proceedings of the National Academy of Sciences of the United States of America

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