Quantifying spatial position in a branched structure in immunostained mouse tissue sections

Silja Heilmann, Henrik Semb, Pia Nyeng*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We have developed a protocol to quantify the position of a cell in a branched structure based on two-dimensional microscopy images of tissue sections. Biological branched structures include organs such as the lungs, kidneys, and pancreas. In these organs, cell fate has been correlated with position, based on a qualitative estimate. However, a quantitative means of evaluating the cell position has been lacking. With this protocol, the correlation between cell fate and cell position was measured in mouse embryonic pancreas.
Original languageEnglish
JournalSTAR protocols
ISSN2666-1667
DOIs
Publication statusPublished - 1 Oct 2021

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