Probiotics and the immunological response to infant vaccinations; a double-blind randomized controlled trial

Camilla Adler Sørensen, Eva Fuglsang, Charlotte Sværke Jørgensen, Rikke Pilmann Laursen, Anni Larnkjær, C. Mølgaard, C Ritz, K.F. Michaelsen, Karen Angeliki Krogfelt, Hanne Frøkier

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Objectives: To examine the effect of a combination of probiotics on the antibody response to pneumo-coccal and pertussis vaccination in healthy Danish children, aged 8-14 months, at the time of starting day care. Moreover, the cytokine response to lipopolysaccharide of whole blood was assessed. Methods: A total of 290 children were randomly allocated to receive a combination of Bifidobacterium animalis ssp. lactis and Lactobacillus rhamnosus GG daily for a 6-month intervention period, and blood samples were drawn at the start and end of the study. Specific antibody response towards Streptococcus pneumoniae serotypes and Bordetella pertussis toxin, as well as endotoxin-induced interleukin-6 (IL-6)and interferon-g(IFN-g) production in blood were analysed by Luminex and ELISA. Results: There was no significant difference between the average individual changes from baseline to endof study in antibody concentrations for S. pneumoniae for both the probiotics (340.4%±11.2%) and the placebo group (382.9%±10.4%) (p 0.525), nor for B. pertussis toxin in the two groups (probiotics190.1%±12.6% versus placebo 238.8%±1.1%, p 0.340). The average individual change in IL-6 concentration was significantly lower in the probiotics versus the placebo group (2.9%±10.3% versus33.7%±9.0%, p 0.024), whereas there was no difference in IFN-g concentration (0.0%±0.2% versuse0.2%±0.1%, p 0.279). Conclusions: The probiotic intervention did not affect the antibody response against S. pneumoniae and B. pertussis toxin in healthy Danish children.
Original languageEnglish
JournalClinical Microbiology and Infection
Issue number4
Number of pages7
Publication statusPublished - 2019

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