New insights into the antimicrobial action of cinnamaldehyde towards escherichia coli and its effects on intestinal colonization of mice

Wellison A. Pereira, Carlos Drielson S. Pereira, Raíssa G. Assunção, Iandeyara Savanna C. da Silva, Fabrícia S. Rego, Leylane S.R. Alves, Juliana S. Santos, Francisco Jonathas R. Nogueira, Adrielle Zagmignan, Thomas T. Thomsen, Anders Løbner-Olesen, Karen A. Krogfelt, Luís Cláudio N. da Silva, Afonso G. Abreu*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

Abstract

Escherichia coli is responsible for cases of diarrhea around the world, and some studies have shown the benefits of cinnamaldehyde in the treatment of bacterial disease. Therefore, the objective of this study was to evaluate the effects of cinnamaldehyde in mice colonized by pathogenic E. coli, as well as to provide more insights into its antimicrobial action mechanism. After determination of minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations, the interference of cinnamaldehyde in macromolecular pathways (synthesis of DNA, RNA, protein, and cell wall) was measured by incorporation of radioisotopes. The anti-adhesive properties of cinnamaldehyde towards E. coli 042 were evaluated using human epithelial type 2 (HEp-2) cells. Intestinal colonization was tested on mice, and the effect of cinnamaldehyde on Tenebrio molitor larvae. Cinnamaldehyde showed MIC and MBC values of 780 µg/mL and 1560 µg/mL, respectively; reduced the adhesion of E. coli 042 on HEp-2 cells; and affected all the synthetic pathways evaluated, suggesting that compost impairs the membrane/cell wall structure leading bacteria to total collapse. No effect on the expression of genes related to the SOS pathway (sulA and dinB1) was observed. The com-pound did not interfere with cell viability and was not toxic against T. molitor larvae. In addition, cinnamaldehyde-treated mice exhibited lower levels of colonization by E. coli 042 than the untreated group. Therefore, the results show that cinnamaldehyde is effective in treating the pathogenic E. coli strain 042 and confirm it as a promising lead molecule for the development of antimicrobial agents.

Original languageEnglish
Article number302
JournalBiomolecules
Volume11
Issue number2
Pages (from-to)1-14
Number of pages14
ISSN2218-273X
DOIs
Publication statusPublished - Feb 2021

Keywords

  • Cinnamaldehyde
  • Intestinal colonization
  • Natural products

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