Localization of GroEL determined by in vivo incorporation of a fluorescent amino acid

Godefroid Charbon, Jiangyun Wang, Eric Brustad, Peter G. Schultz, Arthur L. Horwich, Christine Jacobs-Wagner, Eli Chapman

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The molecular chaperone GroEL is required for bacterial growth under all conditions, mediating folding assistance, via its central cavity, to a diverse set of cytosolic proteins; yet the subcellular localization of GroEL remains unresolved. An earlier study, using antibody probing of fixed Escherichia coli cells, indicated colocalization with the cell division protein FtsZ at the cleavage furrow, while a second E. coli study of fixed cells indicated more even distribution throughout the cytoplasm. Here, for the first time, we have examined the spatial distribution of GroEL in living cells using incorporation of a fluorescent unnatural amino acid into the chaperone. Fluorescence microscopy indicated that GroEL is diffusely distributed, both under normal and stress conditions. Importantly, the present procedure uses a small, fluorescent unnatural amino acid to visualize GroEL in vivo, avoiding the steric demands of a fluorescent protein fusion, which compromises proper GroEL assembly. Further, this unnatural amino acid incorporation avoids artifacts that can occur with fixation and antibody staining.
    Original languageEnglish
    JournalBioorganic & Medicinal Chemistry Letters
    Volume21
    Issue number20
    Pages (from-to)6067-70
    ISSN0960-894X
    DOIs
    Publication statusPublished - 15 Oct 2011

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