Abstract
Introduction
Ulcerative colitis (UC) is a relapsing non-transmural inflammatory disease that is restricted to the colon and is characterized by flare-ups of bloody diarrhea. In this study we aim to investigate intestinal bacterial diversity in healthy controls and patients with UC with and without active disease, from Ghana and Denmark.
Materials and method
This study included 18 healthy controls, 9 UC patients with active and 9 with inactive disease from Ghana. Additionally, 19 healthy controls, 8 UC patients with active and 8 UC patients with inactive disease from Denmark were included in the study. Microbiota diversity analysis relied on sequencing of ribosomal small subunit genes. Purified genomic DNA was submitted to PCR using a primer set targeting prokaryotes and eukaryotes. The purified DNA was sequenced on the Illumina MiSeq system in a 2 × 250 bp set up, Illumina. Analysis of the taxonomy-table was performed blinded using BioNumerics-7.5, Applied Maths.
Results
When analysing the taxonomy-results for prokaryotes and eukaryotes, cluster and principal component analysis shows Danish healthy controls clustered together, but separate from healthy controls from Ghana, which also clustered together.
When analyzing prokaryotes taxonomy-results for Shannon diversity index (SDI), there are significant differences between Danish healthy controls and patients (mean 2-2.7) in comparison to the corresponding groups from Ghana (mean 1.5-1.8), p= 0.0056. The SDI of the eukaryotes ranges between 0-3.1 in the Ghana group, while in the corresponding Danish group it ranges between 2.4-3.2, the difference is non-significant (p=0.138).
Conclusion
Overall, healthy controls and patients with UC from Denmark have increased intestinal prokaryotic and eukaryotic diversity in comparison to corresponding groups from Ghana. Increased variance of the SDI in the Ghana group explains increased differences between individuals in their intestinal microbiota in comparison to the Danish group. Danish UC patients
have decreased variance of the SDI in comparison to Danish controls.
Ulcerative colitis (UC) is a relapsing non-transmural inflammatory disease that is restricted to the colon and is characterized by flare-ups of bloody diarrhea. In this study we aim to investigate intestinal bacterial diversity in healthy controls and patients with UC with and without active disease, from Ghana and Denmark.
Materials and method
This study included 18 healthy controls, 9 UC patients with active and 9 with inactive disease from Ghana. Additionally, 19 healthy controls, 8 UC patients with active and 8 UC patients with inactive disease from Denmark were included in the study. Microbiota diversity analysis relied on sequencing of ribosomal small subunit genes. Purified genomic DNA was submitted to PCR using a primer set targeting prokaryotes and eukaryotes. The purified DNA was sequenced on the Illumina MiSeq system in a 2 × 250 bp set up, Illumina. Analysis of the taxonomy-table was performed blinded using BioNumerics-7.5, Applied Maths.
Results
When analysing the taxonomy-results for prokaryotes and eukaryotes, cluster and principal component analysis shows Danish healthy controls clustered together, but separate from healthy controls from Ghana, which also clustered together.
When analyzing prokaryotes taxonomy-results for Shannon diversity index (SDI), there are significant differences between Danish healthy controls and patients (mean 2-2.7) in comparison to the corresponding groups from Ghana (mean 1.5-1.8), p= 0.0056. The SDI of the eukaryotes ranges between 0-3.1 in the Ghana group, while in the corresponding Danish group it ranges between 2.4-3.2, the difference is non-significant (p=0.138).
Conclusion
Overall, healthy controls and patients with UC from Denmark have increased intestinal prokaryotic and eukaryotic diversity in comparison to corresponding groups from Ghana. Increased variance of the SDI in the Ghana group explains increased differences between individuals in their intestinal microbiota in comparison to the Danish group. Danish UC patients
have decreased variance of the SDI in comparison to Danish controls.
| Translated title of the contribution | Tarm mikrobiota i patienter med Ulcerative colitis og raske kontroler fra Ghana og Danmark |
|---|---|
| Original language | English |
| Publication date | 15 Oct 2021 |
| Number of pages | 1 |
| Publication status | Published - 15 Oct 2021 |
| Event | 12th International Symposium on Gut Microbiology - Virtual Congress Duration: 13 Oct 2021 → 15 Oct 2021 |
Symposium
| Symposium | 12th International Symposium on Gut Microbiology |
|---|---|
| Location | Virtual Congress |
| Period | 13/10/2021 → 15/10/2021 |