HNF1α activates the aminopeptidase N promoter in intestinal (Caco-2) cells

Jørgen Olsen*, Liselotte Laustsen, Jesper Troelsen

*Corresponding author

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1α expression vector demonstrates a direct activation of the promoter by HNF1α through this element. Electrophoretic mobility shift assays using nuclear extracts from Caco-2 cells show the presence of high amounts of HNF1 binding proteins irrespective of their state of differentiation.

Original languageEnglish
JournalFEBS Letters
Volume342
Issue number3
Pages (from-to)325-328
Number of pages4
ISSN0014-5793
DOIs
Publication statusPublished - 11 Apr 1994
Externally publishedYes

Keywords

  • Aminopeptidase N
  • Differentiation
  • HNF1
  • Transcription factor dimerization
  • Transcriptional regulation

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