Global transcriptomic response of the AI-3 isomers 3,5-DPO and 3,6-DPO in Salmonella Typhimurium

Claire Lallement, William Goldring, Lotte Jelsbak*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Bacterial intercellular signaling mediated by small molecules, also called autoinducers (AIs), enables synchronized behavior in response to environmental conditions, and in many bacterial pathogens, intercellular signaling controls virulence gene expression. However, in the intestinal pathogen Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium), although three signals, named AI-1, AI-2 and AI-3, have been described, their roles in virulence remain elusive. AI-3 is the 3,6- isomer of a previously described Vibrio cholerae signaling molecule; 3,5-dimethylpyrazin-2-ol (3,5-DPO). To elucidate the role of AI-3/DPO in S. Typhimurium, we have mapped the global transcriptomic responses to 3,5- and 3,6-DPO isomers in S. Typhimurium. Our studies showed that DPO affects expression of almost 8% of all genes. Specifically, expression of several genes involved in gut-colonization respond to DPO. Interestingly, most of the affected genes are similarly regulated by 3,5-DPO and 3,6-DPO, respectively, indicating that the two isomers have overlapping roles in S. Typhimurium.
Original languageEnglish
Article number117
JournalArchives of microbiology
Volume205
Issue number4
Number of pages7
ISSN0302-8933
DOIs
Publication statusPublished - Apr 2023

Bibliographical note

The datasets generated during and/or analyzed dur-
ing the current study are available in Sequence Read Archive under
Bioproject accession number PRJNA923488

Keywords

  • AI-3
  • DPO
  • Enteric bacteria
  • Quorum sensing
  • RNA-seq

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