Food transfer and uptake of nanosized polystyrene particles in a two-species marine system

Nikoline Bang Oturai, Anita Mammen, Laura Rowenczyk, Alexandra Ter Halle, Ole Vang, Kristian Syberg

Research output: Contribution to conferencePosterResearch

Abstract

Research has shown that smaller size plastic particles (<100nm) are available for biological uptake. Present study investigates the food transfer of polystyrene nanoparticles (50nm) in a maritime set up with Mytilus edulis and Carcinus maenas. Inspired by Dawson et al (2018), underlining the grinding function of krill to turn microplastic (MP) into nanoplastic (NP), the same two-species set-up was exposed to microplastic polystyrene particles (2μm) to investigate the internal fate of microplastics in C. maenas The study aims to assess the importance of food as source for plastic uptake and to what extend translocation occurr in both organisms. Seventy M. edulis were exposed through their diet to two concentrations, 0.167 mg/L and 1.67 mg/L, of polystyrene particles in two sizes, 2μm and 50nm. 35 females of C. maenas were fed one M. edulis apiece. Three tissue samples for the crabs and three for the mussels were analysed by fluorescent absorbance in an ELISA microplate reader. For the nanoplastic particles, we found a transfer from M. edulis to C. maenas for the high exposure treatment as well as for the low exposure treatment, however solely to the stomach tissue. The measurements for the microplastic particles suggested presence of titrated particles (<100nm) in the hepatopancreas. Notably, all p-values showed non-significance, which is likely because of the large background noise as seen in the control treatment. In the recent study by Schür et al (2019) the common methodology of uptake and translocation studies using fluorescent plastic particles is questioned. Results show that leaching of fluorescent dye occurs in lipid droplets in D. magna, and thus suggest artefacts in detection measurements. Further studies are needed to further explore the implications of our findings, e.g. detection confirmation by confocal microscopy.
Original languageEnglish
Publication date2019
Publication statusPublished - 2019
EventSETAC Europe 29th Annual Meeting: One Environment. One Health. Sustainable Societies. - Messukeskus Helsinki, Expo and Convention Centre., Helsinki, Finland
Duration: 26 May 201930 May 2019
https://helsinki.setac.org/

Conference

ConferenceSETAC Europe 29th Annual Meeting
LocationMessukeskus Helsinki, Expo and Convention Centre.
CountryFinland
CityHelsinki
Period26/05/201930/05/2019
Internet address

Cite this

Oturai, N. B., Mammen, A., Rowenczyk, L., Ter Halle, A., Vang, O., & Syberg, K. (2019). Food transfer and uptake of nanosized polystyrene particles in a two-species marine system. Poster session presented at SETAC Europe 29th Annual Meeting, Helsinki, Finland.
Oturai, Nikoline Bang ; Mammen, Anita ; Rowenczyk, Laura ; Ter Halle, Alexandra ; Vang, Ole ; Syberg, Kristian. / Food transfer and uptake of nanosized polystyrene particles in a two-species marine system. Poster session presented at SETAC Europe 29th Annual Meeting, Helsinki, Finland.
@conference{42172bbde35a40fe83470455bb36d617,
title = "Food transfer and uptake of nanosized polystyrene particles in a two-species marine system",
abstract = "Research has shown that smaller size plastic particles (<100nm) are available for biological uptake. Present study investigates the food transfer of polystyrene nanoparticles (50nm) in a maritime set up with Mytilus edulis and Carcinus maenas. Inspired by Dawson et al (2018), underlining the grinding function of krill to turn microplastic (MP) into nanoplastic (NP), the same two-species set-up was exposed to microplastic polystyrene particles (2μm) to investigate the internal fate of microplastics in C. maenas The study aims to assess the importance of food as source for plastic uptake and to what extend translocation occurr in both organisms. Seventy M. edulis were exposed through their diet to two concentrations, 0.167 mg/L and 1.67 mg/L, of polystyrene particles in two sizes, 2μm and 50nm. 35 females of C. maenas were fed one M. edulis apiece. Three tissue samples for the crabs and three for the mussels were analysed by fluorescent absorbance in an ELISA microplate reader. For the nanoplastic particles, we found a transfer from M. edulis to C. maenas for the high exposure treatment as well as for the low exposure treatment, however solely to the stomach tissue. The measurements for the microplastic particles suggested presence of titrated particles (<100nm) in the hepatopancreas. Notably, all p-values showed non-significance, which is likely because of the large background noise as seen in the control treatment. In the recent study by Sch{\"u}r et al (2019) the common methodology of uptake and translocation studies using fluorescent plastic particles is questioned. Results show that leaching of fluorescent dye occurs in lipid droplets in D. magna, and thus suggest artefacts in detection measurements. Further studies are needed to further explore the implications of our findings, e.g. detection confirmation by confocal microscopy.",
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year = "2019",
language = "English",
note = "SETAC Europe 29th Annual Meeting : One Environment. One Health. Sustainable Societies. ; Conference date: 26-05-2019 Through 30-05-2019",
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Oturai, NB, Mammen, A, Rowenczyk, L, Ter Halle, A, Vang, O & Syberg, K 2019, 'Food transfer and uptake of nanosized polystyrene particles in a two-species marine system' SETAC Europe 29th Annual Meeting, Helsinki, Finland, 26/05/2019 - 30/05/2019, .

Food transfer and uptake of nanosized polystyrene particles in a two-species marine system. / Oturai, Nikoline Bang; Mammen, Anita; Rowenczyk, Laura; Ter Halle, Alexandra; Vang, Ole; Syberg, Kristian.

2019. Poster session presented at SETAC Europe 29th Annual Meeting, Helsinki, Finland.

Research output: Contribution to conferencePosterResearch

TY - CONF

T1 - Food transfer and uptake of nanosized polystyrene particles in a two-species marine system

AU - Oturai, Nikoline Bang

AU - Mammen, Anita

AU - Rowenczyk, Laura

AU - Ter Halle, Alexandra

AU - Vang, Ole

AU - Syberg, Kristian

PY - 2019

Y1 - 2019

N2 - Research has shown that smaller size plastic particles (<100nm) are available for biological uptake. Present study investigates the food transfer of polystyrene nanoparticles (50nm) in a maritime set up with Mytilus edulis and Carcinus maenas. Inspired by Dawson et al (2018), underlining the grinding function of krill to turn microplastic (MP) into nanoplastic (NP), the same two-species set-up was exposed to microplastic polystyrene particles (2μm) to investigate the internal fate of microplastics in C. maenas The study aims to assess the importance of food as source for plastic uptake and to what extend translocation occurr in both organisms. Seventy M. edulis were exposed through their diet to two concentrations, 0.167 mg/L and 1.67 mg/L, of polystyrene particles in two sizes, 2μm and 50nm. 35 females of C. maenas were fed one M. edulis apiece. Three tissue samples for the crabs and three for the mussels were analysed by fluorescent absorbance in an ELISA microplate reader. For the nanoplastic particles, we found a transfer from M. edulis to C. maenas for the high exposure treatment as well as for the low exposure treatment, however solely to the stomach tissue. The measurements for the microplastic particles suggested presence of titrated particles (<100nm) in the hepatopancreas. Notably, all p-values showed non-significance, which is likely because of the large background noise as seen in the control treatment. In the recent study by Schür et al (2019) the common methodology of uptake and translocation studies using fluorescent plastic particles is questioned. Results show that leaching of fluorescent dye occurs in lipid droplets in D. magna, and thus suggest artefacts in detection measurements. Further studies are needed to further explore the implications of our findings, e.g. detection confirmation by confocal microscopy.

AB - Research has shown that smaller size plastic particles (<100nm) are available for biological uptake. Present study investigates the food transfer of polystyrene nanoparticles (50nm) in a maritime set up with Mytilus edulis and Carcinus maenas. Inspired by Dawson et al (2018), underlining the grinding function of krill to turn microplastic (MP) into nanoplastic (NP), the same two-species set-up was exposed to microplastic polystyrene particles (2μm) to investigate the internal fate of microplastics in C. maenas The study aims to assess the importance of food as source for plastic uptake and to what extend translocation occurr in both organisms. Seventy M. edulis were exposed through their diet to two concentrations, 0.167 mg/L and 1.67 mg/L, of polystyrene particles in two sizes, 2μm and 50nm. 35 females of C. maenas were fed one M. edulis apiece. Three tissue samples for the crabs and three for the mussels were analysed by fluorescent absorbance in an ELISA microplate reader. For the nanoplastic particles, we found a transfer from M. edulis to C. maenas for the high exposure treatment as well as for the low exposure treatment, however solely to the stomach tissue. The measurements for the microplastic particles suggested presence of titrated particles (<100nm) in the hepatopancreas. Notably, all p-values showed non-significance, which is likely because of the large background noise as seen in the control treatment. In the recent study by Schür et al (2019) the common methodology of uptake and translocation studies using fluorescent plastic particles is questioned. Results show that leaching of fluorescent dye occurs in lipid droplets in D. magna, and thus suggest artefacts in detection measurements. Further studies are needed to further explore the implications of our findings, e.g. detection confirmation by confocal microscopy.

M3 - Poster

ER -

Oturai NB, Mammen A, Rowenczyk L, Ter Halle A, Vang O, Syberg K. Food transfer and uptake of nanosized polystyrene particles in a two-species marine system. 2019. Poster session presented at SETAC Europe 29th Annual Meeting, Helsinki, Finland.