Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo

Pia Nyeng*, Gelo Victoriano Dela Cruz, Henrik Semb

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review


We here report a flow-cytometry-based protocol to measure single-cell protein expression in small samples. The protocol is optimized for simultaneous detection of fluorescent proteins and intracellular and surface antigens in the embryonic pancreas from the mouse. Owing to low cell numbers, current protocols for flow cytometric analysis of embryonic tissues rely on tissue pooling. Our protocol enables analysis of one pancreas per sample, thereby facilitating detection of biological variation and minimizing the number of experimental animals needed. For complete details on the use and execution of this protocol, please refer to Nyeng et al (2019).

Original languageEnglish
Article number100636
JournalSTAR protocols
Issue number3
Pages (from-to)100636
Number of pages1
Publication statusPublished - 17 Sept 2021


  • Cell isolation
  • Developmental biology
  • Flow Cytometry/Mass Cytometry
  • Molecular Biology
  • Single Cell

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