Excess post-hypoxic oxygen consumption is independent from lactate accumulation in two cyprinid fishes

Janet Genz, Mads Jyde, Jon C. Svendsen, John F. Steffensen, Hans Ramløv

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Carassius carassius responds to hypoxic conditions by conversion of lactate into ethanol, which is excreted over the gills. However, a closely related species, Cyprinus carpio, does not possess the ability to produce ethanol and would be expected to accumulate lactate during hypoxic exposure. While the increase in oxygen consumption in fish required following strenuous exercise or low environmental oxygen availability has been frequently considered, the primary contributing mechanism remains unknown. This study utilized the close relationship but strongly divergent physiology between C. carpio and C. carassius to examine the possible correlation between excess post-hypoxic oxygen consumption (EPHOC) and lactate accumulation. No difference in the EPHOC:O2 deficit ratio was observed between the two species after 2.5 h anoxia, with ratios of 2.0 ± 0.6 (C. carpio) and 1.3 ± 0.3 (C. carassius). As predicted, lactate accumulation dynamics did significantly differ between the species in both plasma and white muscle following anoxic exposure. Significant lactate accumulation was seen in both plasma and muscle in C. carpio, but there was no accumulation of lactate in white muscle tissue of C. carassius. These findings indicate that lactate accumulated as a consequence of 2.5 h anoxic exposure is not a major determinant of the resulting EPHOC.
    Original languageEnglish
    JournalComparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology
    Volume165
    Issue number1
    Pages (from-to)54-60
    Number of pages7
    ISSN1095-6433
    DOIs
    Publication statusPublished - 2013

    Cite this

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    title = "Excess post-hypoxic oxygen consumption is independent from lactate accumulation in two cyprinid fishes",
    abstract = "Carassius carassius responds to hypoxic conditions by conversion of lactate into ethanol, which is excreted over the gills. However, a closely related species, Cyprinus carpio, does not possess the ability to produce ethanol and would be expected to accumulate lactate during hypoxic exposure. While the increase in oxygen consumption in fish required following strenuous exercise or low environmental oxygen availability has been frequently considered, the primary contributing mechanism remains unknown. This study utilized the close relationship but strongly divergent physiology between C. carpio and C. carassius to examine the possible correlation between excess post-hypoxic oxygen consumption (EPHOC) and lactate accumulation. No difference in the EPHOC:O2 deficit ratio was observed between the two species after 2.5 h anoxia, with ratios of 2.0 ± 0.6 (C. carpio) and 1.3 ± 0.3 (C. carassius). As predicted, lactate accumulation dynamics did significantly differ between the species in both plasma and white muscle following anoxic exposure. Significant lactate accumulation was seen in both plasma and muscle in C. carpio, but there was no accumulation of lactate in white muscle tissue of C. carassius. These findings indicate that lactate accumulated as a consequence of 2.5 h anoxic exposure is not a major determinant of the resulting EPHOC.",
    keywords = "lactate cyprinid fish",
    author = "Janet Genz and Mads Jyde and Svendsen, {Jon C.} and Steffensen, {John F.} and Hans Raml{\o}v",
    year = "2013",
    doi = "10.1016/j.cbpa.2013.02.002",
    language = "English",
    volume = "165",
    pages = "54--60",
    journal = "Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology",
    issn = "1095-6433",
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    }

    Excess post-hypoxic oxygen consumption is independent from lactate accumulation in two cyprinid fishes. / Genz, Janet; Jyde, Mads; Svendsen, Jon C.; Steffensen, John F.; Ramløv, Hans.

    In: Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, Vol. 165, No. 1, 2013, p. 54-60.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Excess post-hypoxic oxygen consumption is independent from lactate accumulation in two cyprinid fishes

    AU - Genz, Janet

    AU - Jyde, Mads

    AU - Svendsen, Jon C.

    AU - Steffensen, John F.

    AU - Ramløv, Hans

    PY - 2013

    Y1 - 2013

    N2 - Carassius carassius responds to hypoxic conditions by conversion of lactate into ethanol, which is excreted over the gills. However, a closely related species, Cyprinus carpio, does not possess the ability to produce ethanol and would be expected to accumulate lactate during hypoxic exposure. While the increase in oxygen consumption in fish required following strenuous exercise or low environmental oxygen availability has been frequently considered, the primary contributing mechanism remains unknown. This study utilized the close relationship but strongly divergent physiology between C. carpio and C. carassius to examine the possible correlation between excess post-hypoxic oxygen consumption (EPHOC) and lactate accumulation. No difference in the EPHOC:O2 deficit ratio was observed between the two species after 2.5 h anoxia, with ratios of 2.0 ± 0.6 (C. carpio) and 1.3 ± 0.3 (C. carassius). As predicted, lactate accumulation dynamics did significantly differ between the species in both plasma and white muscle following anoxic exposure. Significant lactate accumulation was seen in both plasma and muscle in C. carpio, but there was no accumulation of lactate in white muscle tissue of C. carassius. These findings indicate that lactate accumulated as a consequence of 2.5 h anoxic exposure is not a major determinant of the resulting EPHOC.

    AB - Carassius carassius responds to hypoxic conditions by conversion of lactate into ethanol, which is excreted over the gills. However, a closely related species, Cyprinus carpio, does not possess the ability to produce ethanol and would be expected to accumulate lactate during hypoxic exposure. While the increase in oxygen consumption in fish required following strenuous exercise or low environmental oxygen availability has been frequently considered, the primary contributing mechanism remains unknown. This study utilized the close relationship but strongly divergent physiology between C. carpio and C. carassius to examine the possible correlation between excess post-hypoxic oxygen consumption (EPHOC) and lactate accumulation. No difference in the EPHOC:O2 deficit ratio was observed between the two species after 2.5 h anoxia, with ratios of 2.0 ± 0.6 (C. carpio) and 1.3 ± 0.3 (C. carassius). As predicted, lactate accumulation dynamics did significantly differ between the species in both plasma and white muscle following anoxic exposure. Significant lactate accumulation was seen in both plasma and muscle in C. carpio, but there was no accumulation of lactate in white muscle tissue of C. carassius. These findings indicate that lactate accumulated as a consequence of 2.5 h anoxic exposure is not a major determinant of the resulting EPHOC.

    KW - lactate cyprinid fish

    U2 - 10.1016/j.cbpa.2013.02.002

    DO - 10.1016/j.cbpa.2013.02.002

    M3 - Journal article

    VL - 165

    SP - 54

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    JO - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

    JF - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

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