Evaluating 6 ricin field detection assays

Hans Christian Slotved*, Nadja Sparding, Julia Tanas Tanassi, Nina R. Steenhard, Niels H.H. Heegaard

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

This study presents data showing the performance of 6 commercial detection assays against ricin around concentrations specified as detection limits by the producers. A 2-fold dilution series of 20 ng/ml ricin was prepared and used for testing the lateral-flow kits: BADD, Pro Strips™, ENVI, RAID DX, Ricin BioThreat Alert, and IMASS™ device. Three of the 6 tested field assays (IMASS™ device, ENVI assay, and the BioThreat Alert assay) were able to detect ricin, although differences in the measured detection limits compared to the official detection limits and false-negative results were observed. We were not able to get the BADD, Pro Strips™, and RAID assays to function in our laboratory. We conclude that when purchasing a field responder assay, there is large variation in the specificity of the assays, and a number of in-house tests must be performed to ensure functionality.

Original languageEnglish
JournalBiosecurity and Bioterrorism
Volume12
Issue number4
Pages (from-to)186-189
Number of pages4
ISSN1538-7135
DOIs
Publication statusPublished - 1 Aug 2014
Externally publishedYes

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