Enzymatic Regulation of Cytosolic Thymidine Kinase 1 and Mitochondrial Thymidine Kinase 2

A Mini Review

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The central enzyme on the de novo pathway for synthesis of DNA precursors, the deoxyribonucleoside triphosphates, is ribonucleotide reductase (RNR). Deoxythymidine triphosphate (dTTP) has a key role in control of RNR activity shifting the specificity from pyrimidine to purine nucleotide reduction. Apart from the complex de novo synthesis of dTTP through UDP reduction, dTTP is provided through salvage of thymidine catalyzed by the thymidine kinases, the cytosolic and cell cycle regulated TK1 and the mitochondrial and constitutively expressed TK2. The complex enzymatic regulation of TK1 and TK2 and the possible physiological significance of this regulation will be discussed.
    Original languageEnglish
    JournalNucleosides, Nucleotides and Nucleic Acids
    Volume29
    Issue number4-6
    Pages (from-to)363-369
    Number of pages6
    ISSN1525-7770
    DOIs
    Publication statusPublished - 2010

    Cite this

    @article{ea213d97f73942f59af27dcb65b487bb,
    title = "Enzymatic Regulation of Cytosolic Thymidine Kinase 1 and Mitochondrial Thymidine Kinase 2: A Mini Review",
    abstract = "The central enzyme on the de novo pathway for synthesis of DNA precursors, the deoxyribonucleoside triphosphates, is ribonucleotide reductase (RNR). Deoxythymidine triphosphate (dTTP) has a key role in control of RNR activity shifting the specificity from pyrimidine to purine nucleotide reduction. Apart from the complex de novo synthesis of dTTP through UDP reduction, dTTP is provided through salvage of thymidine catalyzed by the thymidine kinases, the cytosolic and cell cycle regulated TK1 and the mitochondrial and constitutively expressed TK2. The complex enzymatic regulation of TK1 and TK2 and the possible physiological significance of this regulation will be discussed.",
    keywords = "MULATED HUMAN-LYMPHOCYTES, 5'-TRIPHOSPHATE , HUMAN-THYMIDINE-KINASE-1, DNA DEPLETION MYOPATHY, DEOXYRIBONUCLEOSIDE KINASES, SUBSTRATE SPECIFICITIES, KINETIC-PROPERTIES, MOLECULAR-CLONING, DTTP POOL, STABILITY",
    author = "Birgitte Munch-Petersen",
    year = "2010",
    doi = "10.1080/15257771003729591",
    language = "English",
    volume = "29",
    pages = "363--369",
    journal = "Nucleosides, Nucleotides and Nucleic Acids",
    issn = "1525-7770",
    publisher = "Taylor & Francis Inc.",
    number = "4-6",

    }

    Enzymatic Regulation of Cytosolic Thymidine Kinase 1 and Mitochondrial Thymidine Kinase 2 : A Mini Review. / Munch-Petersen, Birgitte.

    In: Nucleosides, Nucleotides and Nucleic Acids, Vol. 29, No. 4-6, 2010, p. 363-369.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Enzymatic Regulation of Cytosolic Thymidine Kinase 1 and Mitochondrial Thymidine Kinase 2

    T2 - A Mini Review

    AU - Munch-Petersen, Birgitte

    PY - 2010

    Y1 - 2010

    N2 - The central enzyme on the de novo pathway for synthesis of DNA precursors, the deoxyribonucleoside triphosphates, is ribonucleotide reductase (RNR). Deoxythymidine triphosphate (dTTP) has a key role in control of RNR activity shifting the specificity from pyrimidine to purine nucleotide reduction. Apart from the complex de novo synthesis of dTTP through UDP reduction, dTTP is provided through salvage of thymidine catalyzed by the thymidine kinases, the cytosolic and cell cycle regulated TK1 and the mitochondrial and constitutively expressed TK2. The complex enzymatic regulation of TK1 and TK2 and the possible physiological significance of this regulation will be discussed.

    AB - The central enzyme on the de novo pathway for synthesis of DNA precursors, the deoxyribonucleoside triphosphates, is ribonucleotide reductase (RNR). Deoxythymidine triphosphate (dTTP) has a key role in control of RNR activity shifting the specificity from pyrimidine to purine nucleotide reduction. Apart from the complex de novo synthesis of dTTP through UDP reduction, dTTP is provided through salvage of thymidine catalyzed by the thymidine kinases, the cytosolic and cell cycle regulated TK1 and the mitochondrial and constitutively expressed TK2. The complex enzymatic regulation of TK1 and TK2 and the possible physiological significance of this regulation will be discussed.

    KW - MULATED HUMAN-LYMPHOCYTES

    KW - 5'-TRIPHOSPHATE

    KW - HUMAN-THYMIDINE-KINASE-1

    KW - DNA DEPLETION MYOPATHY

    KW - DEOXYRIBONUCLEOSIDE KINASES

    KW - SUBSTRATE SPECIFICITIES

    KW - KINETIC-PROPERTIES

    KW - MOLECULAR-CLONING

    KW - DTTP POOL

    KW - STABILITY

    U2 - 10.1080/15257771003729591

    DO - 10.1080/15257771003729591

    M3 - Journal article

    VL - 29

    SP - 363

    EP - 369

    JO - Nucleosides, Nucleotides and Nucleic Acids

    JF - Nucleosides, Nucleotides and Nucleic Acids

    SN - 1525-7770

    IS - 4-6

    ER -