Environmental Metabolism of Pyrene and 1-Methylpyrene by Nereis diversicolor

Linus Mattias Valdemar Malmquist, Jan Henning Christensen, Henriette Selck

Research output: Contribution to conferencePosterResearch

Abstract

Mineral oils from spills contain magnitudes higher proportions of alkylated Polycyclic Aromatic Hydrocarbons (PAHs) compared to unsubstituted PAHs. Despite this, assessments of contaminations are often limited to only consider the “16 USEPA priority PAHs”, which are all unsubstituted. We studied the metabolism of 1-methylpyrene by exposure to the benthic invertebrate Nereis diversicolor in laboratory mesocosms. Analyses of worm, water and sediment extracts were performed by use of Gas Chromatography Mass Spectrometry (GC-MS) and Ultra Performance Liquid Chromatography with UV, Fluorescence and Quantitative Time of Flight Mass Spectrometry detection (UPLC-UV/FLR/QToF). These analyses show formations of known phase II metabolites such as sulphate, glucoronide and glucoside conjugates. However, more than 90% of the metabolites excreted by the organism to water consist of the double benzylic hydroxylated 1COOH-pyrene, not previously reported in relation to ecotoxicology. This metabolite can act as a source to the unsubstituted PAH, pyrene, through environmental decarboxylation processes.
Original languageEnglish
Publication date28 Feb 2011
Number of pages1
Publication statusPublished - 28 Feb 2011
EventYoung Environmental Scientists Meeting (YES-Meeting) - RWTH Aachen University, Aachen, Germany
Duration: 28 Feb 20112 Mar 2011
Conference number: 2
http://yes2011.sac-online.eu/

Conference

ConferenceYoung Environmental Scientists Meeting (YES-Meeting)
Number2
LocationRWTH Aachen University
CountryGermany
CityAachen
Period28/02/201102/03/2011
Internet address

Cite this

Malmquist, L. M. V., Christensen, J. H., & Selck, H. (2011). Environmental Metabolism of Pyrene and 1-Methylpyrene by Nereis diversicolor. Poster session presented at Young Environmental Scientists Meeting (YES-Meeting), Aachen, Germany.
Malmquist, Linus Mattias Valdemar ; Christensen, Jan Henning ; Selck, Henriette. / Environmental Metabolism of Pyrene and 1-Methylpyrene by Nereis diversicolor. Poster session presented at Young Environmental Scientists Meeting (YES-Meeting), Aachen, Germany.1 p.
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title = "Environmental Metabolism of Pyrene and 1-Methylpyrene by Nereis diversicolor",
abstract = "Mineral oils from spills contain magnitudes higher proportions of alkylated Polycyclic Aromatic Hydrocarbons (PAHs) compared to unsubstituted PAHs. Despite this, assessments of contaminations are often limited to only consider the “16 USEPA priority PAHs”, which are all unsubstituted. We studied the metabolism of 1-methylpyrene by exposure to the benthic invertebrate Nereis diversicolor in laboratory mesocosms. Analyses of worm, water and sediment extracts were performed by use of Gas Chromatography Mass Spectrometry (GC-MS) and Ultra Performance Liquid Chromatography with UV, Fluorescence and Quantitative Time of Flight Mass Spectrometry detection (UPLC-UV/FLR/QToF). These analyses show formations of known phase II metabolites such as sulphate, glucoronide and glucoside conjugates. However, more than 90{\%} of the metabolites excreted by the organism to water consist of the double benzylic hydroxylated 1COOH-pyrene, not previously reported in relation to ecotoxicology. This metabolite can act as a source to the unsubstituted PAH, pyrene, through environmental decarboxylation processes.",
keywords = "Alkylated PAHs, 1-methylpyrene, Nereis diversicolor, metabolism, decarboxylation, UPLC, fluorescence, mass spectrometry, Time of Flight",
author = "Malmquist, {Linus Mattias Valdemar} and Christensen, {Jan Henning} and Henriette Selck",
year = "2011",
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day = "28",
language = "English",
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Malmquist, LMV, Christensen, JH & Selck, H 2011, 'Environmental Metabolism of Pyrene and 1-Methylpyrene by Nereis diversicolor', Aachen, Germany, 28/02/2011 - 02/03/2011, .

Environmental Metabolism of Pyrene and 1-Methylpyrene by Nereis diversicolor. / Malmquist, Linus Mattias Valdemar; Christensen, Jan Henning; Selck, Henriette.

2011. Poster session presented at Young Environmental Scientists Meeting (YES-Meeting), Aachen, Germany.

Research output: Contribution to conferencePosterResearch

TY - CONF

T1 - Environmental Metabolism of Pyrene and 1-Methylpyrene by Nereis diversicolor

AU - Malmquist, Linus Mattias Valdemar

AU - Christensen, Jan Henning

AU - Selck, Henriette

PY - 2011/2/28

Y1 - 2011/2/28

N2 - Mineral oils from spills contain magnitudes higher proportions of alkylated Polycyclic Aromatic Hydrocarbons (PAHs) compared to unsubstituted PAHs. Despite this, assessments of contaminations are often limited to only consider the “16 USEPA priority PAHs”, which are all unsubstituted. We studied the metabolism of 1-methylpyrene by exposure to the benthic invertebrate Nereis diversicolor in laboratory mesocosms. Analyses of worm, water and sediment extracts were performed by use of Gas Chromatography Mass Spectrometry (GC-MS) and Ultra Performance Liquid Chromatography with UV, Fluorescence and Quantitative Time of Flight Mass Spectrometry detection (UPLC-UV/FLR/QToF). These analyses show formations of known phase II metabolites such as sulphate, glucoronide and glucoside conjugates. However, more than 90% of the metabolites excreted by the organism to water consist of the double benzylic hydroxylated 1COOH-pyrene, not previously reported in relation to ecotoxicology. This metabolite can act as a source to the unsubstituted PAH, pyrene, through environmental decarboxylation processes.

AB - Mineral oils from spills contain magnitudes higher proportions of alkylated Polycyclic Aromatic Hydrocarbons (PAHs) compared to unsubstituted PAHs. Despite this, assessments of contaminations are often limited to only consider the “16 USEPA priority PAHs”, which are all unsubstituted. We studied the metabolism of 1-methylpyrene by exposure to the benthic invertebrate Nereis diversicolor in laboratory mesocosms. Analyses of worm, water and sediment extracts were performed by use of Gas Chromatography Mass Spectrometry (GC-MS) and Ultra Performance Liquid Chromatography with UV, Fluorescence and Quantitative Time of Flight Mass Spectrometry detection (UPLC-UV/FLR/QToF). These analyses show formations of known phase II metabolites such as sulphate, glucoronide and glucoside conjugates. However, more than 90% of the metabolites excreted by the organism to water consist of the double benzylic hydroxylated 1COOH-pyrene, not previously reported in relation to ecotoxicology. This metabolite can act as a source to the unsubstituted PAH, pyrene, through environmental decarboxylation processes.

KW - Alkylated PAHs

KW - 1-methylpyrene

KW - Nereis diversicolor

KW - metabolism

KW - decarboxylation

KW - UPLC

KW - fluorescence

KW - mass spectrometry

KW - Time of Flight

M3 - Poster

ER -

Malmquist LMV, Christensen JH, Selck H. Environmental Metabolism of Pyrene and 1-Methylpyrene by Nereis diversicolor. 2011. Poster session presented at Young Environmental Scientists Meeting (YES-Meeting), Aachen, Germany.