Does bisphenol A induce superfeminization in Marisa cornuarietis? Part II

Toxicity test results and requirements for statistical power analyses

Valery E. Forbes, John Aufderheide, Ryan Warbritton, Nelly van der Hoeven, Norbert Caspers

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

This study presents results of the effects of bisphenol A (BPA) on adult egg production, egg hatchability, egg development rates and juvenile growth rates in the freshwater gastropod, Marisa cornuarietis. We observed no adult mortality, substantial inter-snail variability in reproductive output, and no effects of BPA on reproduction during 12 weeks of exposure to 0, 0.1, 1.0, 16, 160 or 640 μg/L BPA. We observed no effects of BPA on egg hatchability or timing of egg hatching. Juveniles showed good growth in the control and all treatments, and there were no significant effects of BPA on this endpoint. Our results do not support previous claims of enhanced reproduction in Marisa cornuarietis in response to exposure to BPA. Statistical power analysis indicated high levels of inter-snail variability in the measured endpoints and highlighted the need for sufficient replication when testing treatment effects on reproduction in M. cornuarietis with adequate power
Original languageEnglish
JournalEcotoxicology and Environmental Safety
Volume66
Issue number3
Pages (from-to)319-325
Number of pages7
ISSN0147-6513
DOIs
Publication statusPublished - 2007

Keywords

  • Endocrine disruption
  • Low-dose effects
  • Reproduction
  • Risk assessment
  • Statistical power
  • Variability

Cite this

Forbes, Valery E. ; Aufderheide, John ; Warbritton, Ryan ; van der Hoeven, Nelly ; Caspers, Norbert. / Does bisphenol A induce superfeminization in Marisa cornuarietis? Part II : Toxicity test results and requirements for statistical power analyses. In: Ecotoxicology and Environmental Safety. 2007 ; Vol. 66, No. 3. pp. 319-325.
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title = "Does bisphenol A induce superfeminization in Marisa cornuarietis? Part II: Toxicity test results and requirements for statistical power analyses",
abstract = "This study presents results of the effects of bisphenol A (BPA) on adult egg production, egg hatchability, egg development rates and juvenile growth rates in the freshwater gastropod, Marisa cornuarietis. We observed no adult mortality, substantial inter-snail variability in reproductive output, and no effects of BPA on reproduction during 12 weeks of exposure to 0, 0.1, 1.0, 16, 160 or 640 μg/L BPA. We observed no effects of BPA on egg hatchability or timing of egg hatching. Juveniles showed good growth in the control and all treatments, and there were no significant effects of BPA on this endpoint. Our results do not support previous claims of enhanced reproduction in Marisa cornuarietis in response to exposure to BPA. Statistical power analysis indicated high levels of inter-snail variability in the measured endpoints and highlighted the need for sufficient replication when testing treatment effects on reproduction in M. cornuarietis with adequate power",
keywords = "Endocrine disruption, Low-dose effects, Reproduction, Risk assessment, Statistical power, Variability",
author = "Forbes, {Valery E.} and John Aufderheide and Ryan Warbritton and {van der Hoeven}, Nelly and Norbert Caspers",
year = "2007",
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Does bisphenol A induce superfeminization in Marisa cornuarietis? Part II : Toxicity test results and requirements for statistical power analyses. / Forbes, Valery E.; Aufderheide, John; Warbritton, Ryan; van der Hoeven, Nelly; Caspers, Norbert.

In: Ecotoxicology and Environmental Safety, Vol. 66, No. 3, 2007, p. 319-325.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Does bisphenol A induce superfeminization in Marisa cornuarietis? Part II

T2 - Toxicity test results and requirements for statistical power analyses

AU - Forbes, Valery E.

AU - Aufderheide, John

AU - Warbritton, Ryan

AU - van der Hoeven, Nelly

AU - Caspers, Norbert

PY - 2007

Y1 - 2007

N2 - This study presents results of the effects of bisphenol A (BPA) on adult egg production, egg hatchability, egg development rates and juvenile growth rates in the freshwater gastropod, Marisa cornuarietis. We observed no adult mortality, substantial inter-snail variability in reproductive output, and no effects of BPA on reproduction during 12 weeks of exposure to 0, 0.1, 1.0, 16, 160 or 640 μg/L BPA. We observed no effects of BPA on egg hatchability or timing of egg hatching. Juveniles showed good growth in the control and all treatments, and there were no significant effects of BPA on this endpoint. Our results do not support previous claims of enhanced reproduction in Marisa cornuarietis in response to exposure to BPA. Statistical power analysis indicated high levels of inter-snail variability in the measured endpoints and highlighted the need for sufficient replication when testing treatment effects on reproduction in M. cornuarietis with adequate power

AB - This study presents results of the effects of bisphenol A (BPA) on adult egg production, egg hatchability, egg development rates and juvenile growth rates in the freshwater gastropod, Marisa cornuarietis. We observed no adult mortality, substantial inter-snail variability in reproductive output, and no effects of BPA on reproduction during 12 weeks of exposure to 0, 0.1, 1.0, 16, 160 or 640 μg/L BPA. We observed no effects of BPA on egg hatchability or timing of egg hatching. Juveniles showed good growth in the control and all treatments, and there were no significant effects of BPA on this endpoint. Our results do not support previous claims of enhanced reproduction in Marisa cornuarietis in response to exposure to BPA. Statistical power analysis indicated high levels of inter-snail variability in the measured endpoints and highlighted the need for sufficient replication when testing treatment effects on reproduction in M. cornuarietis with adequate power

KW - Endocrine disruption

KW - Low-dose effects

KW - Reproduction

KW - Risk assessment

KW - Statistical power

KW - Variability

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DO - doi:10.1016/j.ecoenv.2006.09.001

M3 - Journal article

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JO - Ecotoxicology and Environmental Safety

JF - Ecotoxicology and Environmental Safety

SN - 0147-6513

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ER -