Non-typhoid salmonellosis is one of the most common causes of foodborne illness throughout the world. Serological methods for the diagnosis of Salmonella infections vary widely and the most commonly used test is limited by high running costs as well as low sensitivity and specificity. Fast and reliable immunoassays which detect subunit antigens for Salmonella enterica subsp. enterica serovar Typhi are commercially available but at present there is no international consensus on similar tests for non-typhoid salmonellosis. In contrast to the veterinary and food sectors, most immunoassays for non-typhoid human Salmonella diagnosis are developed in-house and used in-house for research or surveillance purposes, rather than for routine diagnostics. Considering the current burden of disease, the development of a validated and standardized, commercially available antibody assay for diagnosing non-typhoid human salmonellosis could be of great benefit for diagnostic and surveillance purposes throughout the world.