Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosine

Tinkara Tinta, Louise Slot Christiansen, Anke Konrad, David A Liberles, Valentina Turk, Birgitte Munch-Petersen, Jure Piskur, Anders Ranegaard Clausen

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    Abstract

    Deoxyribonucleoside kinases (dNKs) are essential in the mammalian cell but their 'importance' in bacteria, especially aquatic ones, is less clear. We studied two aquatic bacteria, Gram-negative Flavobacterium psychrophilum JIP02/86 and Polaribacter sp. MED152, for their ability to salvage deoxyribonucleosides (dNs). Both had a Gram-positive-type thymidine kinase (TK1), which could phosphorylate thymidine, and one non-TK1 dNK, which could efficiently phosphorylate deoxyadenosine and slightly also deoxycytosine. Surprisingly, the four tested dNKs could not phosphorylate deoxyguanosine, and apparently, these two bacteria are missing this activity. When tens of available aquatic bacteria genomes were examined for the presence of dNKs, a majority had at least a TK1-like gene, but several lacked any dNKs. Apparently, among aquatic bacteria, the role of the dN salvage varies.
    Original languageDanish
    JournalF E M S Microbiology Letters
    Volume331
    Issue number2
    Pages (from-to)120-127
    Number of pages8
    ISSN0378-1097
    DOIs
    Publication statusPublished - Jan 2012

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