Danish Culicoides species of the Obsoletus group identified by morphological methods

Søren Achim Nielsen; M. Kristensen

Danish Culicoides species of the Obsoletus group identified by morphological methods

Søren Achim Nielsen, M. Kristensen

Environmental Dynamics

Research output: Contribution to conference › Conference abstract for conference › Research

Abstract

A method was presented to morphologically separate C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus. From field-collected individuals, hypopygium and wing for male and head, wing, and spermatheca for female were observed and species identification was obtained using DNA assay. Differences between C. obsoletus and C. scoticus were found using the ratio of the length and width of the third segment of the palpus, the antennal ratio (sum of the lengths of the apical five segments of the the flagellum (11-15) to the sum of basal eight) and the wing length. Finally, there were few overlaps crossing the palpus ratio and the wing length.

PCR identification showed a 100% agreement between morphology and molecular identification. ITS1 sequences were slightly variable within a species (less than 1% in C. obsoletus and about 3% in C. scoticus), when differences between C. obsoletus and C scoticus were about 5 to 7%. and about 3% in ), when differences between and were about 5 to 7%.

Conclusion: the importance of crossing different characters is highlighted to identify

species.

Original language	English
Publication date	2009
Number of pages	1
Publication status	Published - 2009
Event	MedReoNet taxonomy meeting - Strasbourg, France Duration: 16 Mar 2009 → 20 Mar 2009

Conference

Conference	MedReoNet taxonomy meeting
Country/Territory	France
City	Strasbourg
Period	16/03/2009 → 20/03/2009

Link to document

http://www.google.dk/url?sa=t&source=web&ct=res&cd=1&ved=0CAYQFjAA&url=http%3A%2F%2Fmedreonet.cirad.fr%2Fcontent%2Fdownload%2F1096%2F5651%2Ffile%2FMedReoNet_Minutes_VFinal.pdf&rct=j&q=avaritia+nielsen+strasbourg&ei=LCuOS-TVIsmQ-AbLyfTkCQ&usg=AFQjCNGVvrrjOVWzfWx9VurEPS7tM0F8sQ

Cite this

Nielsen, S. A., & Kristensen, M. (2009). Danish Culicoides species of the Obsoletus group identified by morphological methods. Abstract from MedReoNet taxonomy meeting, Strasbourg, France. http://www.google.dk/url?sa=t&source=web&ct=res&cd=1&ved=0CAYQFjAA&url=http%3A%2F%2Fmedreonet.cirad.fr%2Fcontent%2Fdownload%2F1096%2F5651%2Ffile%2FMedReoNet_Minutes_VFinal.pdf&rct=j&q=avaritia+nielsen+strasbourg&ei=LCuOS-TVIsmQ-AbLyfTkCQ&usg=AFQjCNGVvrrjOVWzfWx9VurEPS7tM0F8sQ

@conference{4e60872026b111df9df3000ea68e967b,

title = "Danish Culicoides species of the Obsoletus group identified by morphological methods",

abstract = "A method was presented to morphologically separate C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus. From field-collected individuals, hypopygium and wing for male and head, wing, and spermatheca for female were observed and species identification was obtained using DNA assay. Differences between C. obsoletus and C. scoticus were found using the ratio of the length and width of the third segment of the palpus, the antennal ratio (sum of the lengths of the apical five segments of the the flagellum (11-15) to the sum of basal eight) and the wing length. Finally, there were few overlaps crossing the palpus ratio and the wing length. PCR identification showed a 100% agreement between morphology and molecular identification. ITS1 sequences were slightly variable within a species (less than 1% in C. obsoletus and about 3% in C. scoticus), when differences between C. obsoletus and C scoticus were about 5 to 7%. and about 3% in ), when differences between and were about 5 to 7%. Conclusion: the importance of crossing different characters is highlighted to identifyspecies.",

author = "Nielsen, {S{\o}ren Achim} and M. Kristensen",

year = "2009",

language = "English",

note = "MedReoNet taxonomy meeting ; Conference date: 16-03-2009 Through 20-03-2009",

}

Nielsen, SA & Kristensen, M 2009, 'Danish Culicoides species of the Obsoletus group identified by morphological methods', MedReoNet taxonomy meeting, Strasbourg, France, 16/03/2009 - 20/03/2009. <http://www.google.dk/url?sa=t&source=web&ct=res&cd=1&ved=0CAYQFjAA&url=http%3A%2F%2Fmedreonet.cirad.fr%2Fcontent%2Fdownload%2F1096%2F5651%2Ffile%2FMedReoNet_Minutes_VFinal.pdf&rct=j&q=avaritia+nielsen+strasbourg&ei=LCuOS-TVIsmQ-AbLyfTkCQ&usg=AFQjCNGVvrrjOVWzfWx9VurEPS7tM0F8sQ>

TY - ABST

T1 - Danish Culicoides species of the Obsoletus group identified by morphological methods

AU - Nielsen, Søren Achim

AU - Kristensen, M.

PY - 2009

Y1 - 2009

N2 - A method was presented to morphologically separate C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus. From field-collected individuals, hypopygium and wing for male and head, wing, and spermatheca for female were observed and species identification was obtained using DNA assay. Differences between C. obsoletus and C. scoticus were found using the ratio of the length and width of the third segment of the palpus, the antennal ratio (sum of the lengths of the apical five segments of the the flagellum (11-15) to the sum of basal eight) and the wing length. Finally, there were few overlaps crossing the palpus ratio and the wing length. PCR identification showed a 100% agreement between morphology and molecular identification. ITS1 sequences were slightly variable within a species (less than 1% in C. obsoletus and about 3% in C. scoticus), when differences between C. obsoletus and C scoticus were about 5 to 7%. and about 3% in ), when differences between and were about 5 to 7%. Conclusion: the importance of crossing different characters is highlighted to identifyspecies.

AB - A method was presented to morphologically separate C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus. From field-collected individuals, hypopygium and wing for male and head, wing, and spermatheca for female were observed and species identification was obtained using DNA assay. Differences between C. obsoletus and C. scoticus were found using the ratio of the length and width of the third segment of the palpus, the antennal ratio (sum of the lengths of the apical five segments of the the flagellum (11-15) to the sum of basal eight) and the wing length. Finally, there were few overlaps crossing the palpus ratio and the wing length. PCR identification showed a 100% agreement between morphology and molecular identification. ITS1 sequences were slightly variable within a species (less than 1% in C. obsoletus and about 3% in C. scoticus), when differences between C. obsoletus and C scoticus were about 5 to 7%. and about 3% in ), when differences between and were about 5 to 7%. Conclusion: the importance of crossing different characters is highlighted to identifyspecies.

M3 - Conference abstract for conference

T2 - MedReoNet taxonomy meeting

Y2 - 16 March 2009 through 20 March 2009

ER -