Danish Culicoides species of the Obsoletus group identified by morphological methods

Søren Achim Nielsen, M. Kristensen

Research output: Contribution to conferenceConference abstract for conferenceResearch


A method was presented to morphologically separate C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus. From field-collected individuals, hypopygium and wing for male and head, wing, and spermatheca for female were observed and species identification was obtained using DNA assay. Differences between C. obsoletus and C. scoticus were found using the ratio of the length and width of the third segment of the palpus, the antennal ratio (sum of the lengths of the apical five segments of the the flagellum (11-15) to the sum of basal eight) and the wing length. Finally, there were few overlaps crossing the palpus ratio and the wing length.

PCR identification showed a 100% agreement between morphology and molecular identification. ITS1 sequences were slightly variable within a species (less than 1% in C. obsoletus and about 3% in C. scoticus), when differences between C. obsoletus and C scoticus were about 5 to 7%. and about 3% in ), when differences between and were about 5 to 7%.

Conclusion: the importance of crossing different characters is highlighted to identify


Original languageEnglish
Publication date2009
Number of pages1
Publication statusPublished - 2009
EventMedReoNet taxonomy meeting - Strasbourg, France
Duration: 16 Mar 200920 Mar 2009


ConferenceMedReoNet taxonomy meeting

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