Cellular cross talk in the small intestinal mucosa: Postnatal lymphocytic immigration elicits a specific epithelial transcriptional response

Katrine T.B.G. Schjoldager, Henrik R. Maltesen, Sophie Balmer, Leif R. Lund, Mogens H. Claesson, Hans Sjöström, Jesper T. Troelsen, Jørgen Olsen*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

Abstract

During the early postnatal period lymphocytes migrate into the mouse small intestine. Migrating infiltrative lymphocytes have the potential to affect the epithelial cells via secreted cytokines. Such cross talk can result in the elicitation of an epithelial transcriptional response. Knowledge about such physiological cross talk between the immune system and the epithelium in the postnatal small intestinal mucosa is lacking. We have investigated the transcriptome changes occurring in the postnatal mouse small intestine using DNA microarray technology, immunocytochemistry, and quantitative real-time RT-PCR analysis. The DNA microarray data were analyzed bioinformatically by using a combination of projections to latent structures analysis and functional annotation analysis. The results show that infiltrating lymphocytes appear in the mouse small intestine in the late postweaning period and give rise to distinct changes in the epithelial transcriptome. Of particular interest is the expression of three genes encoding a mucin (Muc4), a mucinlike protein (16000D21Rik), and ATP citrate lyase (Acly). All three genes were shown to be expressed by the epithelium and to be upregulated in response to lymphocytic migration into the small intestinal mucosa.

Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume294
Issue number6
Pages (from-to)G1335-G1343
ISSN0193-1857
DOIs
Publication statusPublished - Jun 2008
Externally publishedYes

Keywords

  • DNA microarray
  • Intestinal development
  • Intestinal gene expression
  • Intraepithelial lymphocytes
  • Projections to latent structures

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