(alpha)B-crystallin in cerebrospinal fluid of patients with multiple sclerosis

Birgitte Støvring, Ole Vang, Michael Christiansen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Background: aB-crystallin is a chaperone protein and a potential myelin antigen to human T cells in Multiple Sclerosis (MS). In this study we investigate the existence of aB-crystallin in the cerebrospinal fluid (CSF) of patients with clinical symptoms of MS and control individuals without these symptoms, using a newly developed aB-crystallin specific chicken antibody. Methods: A chicken anti-aB-crystallin was raised against recombinant aB-crystallin, characterized and used in a semiquantitative Western blot analysis of CSF from 16 MS patients and 16 neurological patients without MS. Results: Western blot analysis revealed the presence of high molecular weight aB-crystallin in CSF. Possibly posttranslationally modified aggregates of aB-crystallin were found in human astroglioma U373 cells. CSF aB-crystallin was seen in the CSF in 100% of MS patients and 88% of neurological controls without MS. Conclusion: aB-crystallin is present in the CSF in patients with MS and other neurological controls. Furthermore, aB-crystallin in CSF and human astroglioma cell line U373 is found in aggregates.
    Original languageEnglish
    JournalClinica Chimica Acta
    Volume356
    Issue number1-2
    Pages (from-to)95-101
    ISSN0009-8981
    DOIs
    Publication statusPublished - 2005

    Keywords

    • Polyclonal antibody
    • Autoantigen
    • Cerebrospinal fluid
    • (alpha)B-crystallin
    • Multiple sclerosis

    Cite this

    Støvring, Birgitte ; Vang, Ole ; Christiansen, Michael. / (alpha)B-crystallin in cerebrospinal fluid of patients with multiple sclerosis. In: Clinica Chimica Acta. 2005 ; Vol. 356, No. 1-2. pp. 95-101.
    @article{e543867052ce11db8236000ea68e967b,
    title = "(alpha)B-crystallin in cerebrospinal fluid of patients with multiple sclerosis",
    abstract = "Background: aB-crystallin is a chaperone protein and a potential myelin antigen to human T cells in Multiple Sclerosis (MS). In this study we investigate the existence of aB-crystallin in the cerebrospinal fluid (CSF) of patients with clinical symptoms of MS and control individuals without these symptoms, using a newly developed aB-crystallin specific chicken antibody. Methods: A chicken anti-aB-crystallin was raised against recombinant aB-crystallin, characterized and used in a semiquantitative Western blot analysis of CSF from 16 MS patients and 16 neurological patients without MS. Results: Western blot analysis revealed the presence of high molecular weight aB-crystallin in CSF. Possibly posttranslationally modified aggregates of aB-crystallin were found in human astroglioma U373 cells. CSF aB-crystallin was seen in the CSF in 100{\%} of MS patients and 88{\%} of neurological controls without MS. Conclusion: aB-crystallin is present in the CSF in patients with MS and other neurological controls. Furthermore, aB-crystallin in CSF and human astroglioma cell line U373 is found in aggregates.",
    keywords = "Polyclonal antibody, Autoantigen, Cerebrospinal fluid, (alpha)B-crystallin, Multiple sclerosis",
    author = "Birgitte St{\o}vring and Ole Vang and Michael Christiansen",
    year = "2005",
    doi = "10.1016/j.cccn.2005.01.034",
    language = "English",
    volume = "356",
    pages = "95--101",
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    (alpha)B-crystallin in cerebrospinal fluid of patients with multiple sclerosis. / Støvring, Birgitte; Vang, Ole; Christiansen, Michael.

    In: Clinica Chimica Acta, Vol. 356, No. 1-2, 2005, p. 95-101.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - (alpha)B-crystallin in cerebrospinal fluid of patients with multiple sclerosis

    AU - Støvring, Birgitte

    AU - Vang, Ole

    AU - Christiansen, Michael

    PY - 2005

    Y1 - 2005

    N2 - Background: aB-crystallin is a chaperone protein and a potential myelin antigen to human T cells in Multiple Sclerosis (MS). In this study we investigate the existence of aB-crystallin in the cerebrospinal fluid (CSF) of patients with clinical symptoms of MS and control individuals without these symptoms, using a newly developed aB-crystallin specific chicken antibody. Methods: A chicken anti-aB-crystallin was raised against recombinant aB-crystallin, characterized and used in a semiquantitative Western blot analysis of CSF from 16 MS patients and 16 neurological patients without MS. Results: Western blot analysis revealed the presence of high molecular weight aB-crystallin in CSF. Possibly posttranslationally modified aggregates of aB-crystallin were found in human astroglioma U373 cells. CSF aB-crystallin was seen in the CSF in 100% of MS patients and 88% of neurological controls without MS. Conclusion: aB-crystallin is present in the CSF in patients with MS and other neurological controls. Furthermore, aB-crystallin in CSF and human astroglioma cell line U373 is found in aggregates.

    AB - Background: aB-crystallin is a chaperone protein and a potential myelin antigen to human T cells in Multiple Sclerosis (MS). In this study we investigate the existence of aB-crystallin in the cerebrospinal fluid (CSF) of patients with clinical symptoms of MS and control individuals without these symptoms, using a newly developed aB-crystallin specific chicken antibody. Methods: A chicken anti-aB-crystallin was raised against recombinant aB-crystallin, characterized and used in a semiquantitative Western blot analysis of CSF from 16 MS patients and 16 neurological patients without MS. Results: Western blot analysis revealed the presence of high molecular weight aB-crystallin in CSF. Possibly posttranslationally modified aggregates of aB-crystallin were found in human astroglioma U373 cells. CSF aB-crystallin was seen in the CSF in 100% of MS patients and 88% of neurological controls without MS. Conclusion: aB-crystallin is present in the CSF in patients with MS and other neurological controls. Furthermore, aB-crystallin in CSF and human astroglioma cell line U373 is found in aggregates.

    KW - Polyclonal antibody

    KW - Autoantigen

    KW - Cerebrospinal fluid

    KW - (alpha)B-crystallin

    KW - Multiple sclerosis

    U2 - 10.1016/j.cccn.2005.01.034

    DO - 10.1016/j.cccn.2005.01.034

    M3 - Journal article

    VL - 356

    SP - 95

    EP - 101

    JO - Clinica Chimica Acta

    JF - Clinica Chimica Acta

    SN - 0009-8981

    IS - 1-2

    ER -