Acidic–alkaline ferulic acid esterase from Chaetomium thermophilum var. dissitum: Molecular cloning and characterization of recombinant enzyme expressed in Pichia pastoris

Gleb Dotsenko, Xiaoxue Tong, Bo Pilgaard, Peter Kamp Busk, Lene Lange

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A novel ferulic acid esterase encoding gene CtFae, was successfully cloned from a highly esterase active strain of the thermophile ascomycetous fungus Chaetomium thermophilum var. dissitum; the gene was heterologously expressed in Pichia pastoris KM71H. The recombinant enzyme (CtFae) was purified to homogeneity and subsequently characterized. CtFae was active towards synthetic esters of ferulic, p-coumaric, and caffeic acids, as well as towards wide range of p-nitrophenyl substrates. Its temperature and pH optima were 55 °C and pH 6.0, respectively. Enzyme rare features were broad pH optimum, high stability at extended acidic–alkaline pH region, and noticeable thermostability. CtFae released ferulic acid from wheat insoluble arabinoxylan, as well as ferulic and p-coumaric acids from wheat straw and ryegrass, indicating potentials for industrial applications like biomass conversion in biorefineries.
Original languageEnglish
JournalBiocatalysis and Agricultural Biotechnology
Volume5
Pages (from-to)48-55
Number of pages8
DOIs
Publication statusPublished - 1 Jan 2016
Externally publishedYes

Keywords

  • Ferulic acid esterase
  • Chaetomium thermophilum
  • Hydroxycinnamic acids
  • Arabinoxylan
  • Wheat straw

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