A simple and fast method for extraction and quantification of cryptophyte phycoerythrin

Christina Vinum Thoisen; Benni Winding Hansen; Søren Laurentius Nielsen

doi:10.1016/j.mex.2017.06.002

A simple and fast method for extraction and quantification of cryptophyte phycoerythrin

Christina Vinum Thoisen, Benni Winding Hansen, Søren Laurentius Nielsen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The microalgal pigment phycoerythrin (PE) is of commercial interest as natural colorant in food and cosmetics, as well as fluoroprobes for laboratory analysis. Several methods for extraction and quantification of PE are available but they comprise typically various extraction buffers, repetitive freeze-thaw cycles and liquid nitrogen, making extraction procedures more complicated. A simple method for extraction of PE from cryptophytes is described using standard laboratory materials and equipment. Filters with the cryptophyte were frozen (−80 °C) and added phosphate buffer for extraction at 4 °C followed by absorbance measurement. The cryptophyte Rhodomonas salina was used as a model organism.

•Simple method for extraction and quantification of phycoerythrin from cryptophytes.
•Minimal usage of equipment and chemicals, and low labor costs.
•Applicable for industrial and biological purposes.

Original language	English
Journal	MethodsX
Volume	4
Issue number	C
Pages (from-to)	209-213
Number of pages	5
ISSN	2215-0161
DOIs	https://doi.org/10.1016/j.mex.2017.06.002
Publication status	Published - 28 Jul 2017

Keywords

Labor cost
Microalgae
Phycobiliprotein
Pigment
Processing time
Rhodomonas salina

Link to document

10.1016/j.mex.2017.06.002

1-s2.0-S2215016117300195-mainFinal published version, 330 KBLicence: CC BY

http://www.sciencedirect.com/science/article/pii/S2215016117300195Licence: Unspecified

COMA: COpepod egg Mass production in Aquaculture
Jepsen, P. M., Hansen, B. W., Nielsen, S. L. & Thoisen, C.
30/05/2013 → 04/12/2017
Project: Research

Cite this

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title = "A simple and fast method for extraction and quantification of cryptophyte phycoerythrin",

abstract = "The microalgal pigment phycoerythrin (PE) is of commercial interest as natural colorant in food and cosmetics, as well as fluoroprobes for laboratory analysis. Several methods for extraction and quantification of PE are available but they comprise typically various extraction buffers, repetitive freeze-thaw cycles and liquid nitrogen, making extraction procedures more complicated. A simple method for extraction of PE from cryptophytes is described using standard laboratory materials and equipment. Filters with the cryptophyte were frozen (−80 °C) and added phosphate buffer for extraction at 4 °C followed by absorbance measurement. The cryptophyte Rhodomonas salina was used as a model organism.•Simple method for extraction and quantification of phycoerythrin from cryptophytes.•Minimal usage of equipment and chemicals, and low labor costs.•Applicable for industrial and biological purposes.",

keywords = " Labor cost, Microalgae, Phycobiliprotein, Pigment, Processing time, Rhodomonas salina",

author = "Thoisen, {Christina Vinum} and Hansen, {Benni Winding} and Nielsen, {S{\o}ren Laurentius}",

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doi = "10.1016/j.mex.2017.06.002",

language = "English",

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TY - JOUR

T1 - A simple and fast method for extraction and quantification of cryptophyte phycoerythrin

AU - Thoisen, Christina Vinum

AU - Hansen, Benni Winding

AU - Nielsen, Søren Laurentius

PY - 2017/7/28

Y1 - 2017/7/28

N2 - The microalgal pigment phycoerythrin (PE) is of commercial interest as natural colorant in food and cosmetics, as well as fluoroprobes for laboratory analysis. Several methods for extraction and quantification of PE are available but they comprise typically various extraction buffers, repetitive freeze-thaw cycles and liquid nitrogen, making extraction procedures more complicated. A simple method for extraction of PE from cryptophytes is described using standard laboratory materials and equipment. Filters with the cryptophyte were frozen (−80 °C) and added phosphate buffer for extraction at 4 °C followed by absorbance measurement. The cryptophyte Rhodomonas salina was used as a model organism.•Simple method for extraction and quantification of phycoerythrin from cryptophytes.•Minimal usage of equipment and chemicals, and low labor costs.•Applicable for industrial and biological purposes.

AB - The microalgal pigment phycoerythrin (PE) is of commercial interest as natural colorant in food and cosmetics, as well as fluoroprobes for laboratory analysis. Several methods for extraction and quantification of PE are available but they comprise typically various extraction buffers, repetitive freeze-thaw cycles and liquid nitrogen, making extraction procedures more complicated. A simple method for extraction of PE from cryptophytes is described using standard laboratory materials and equipment. Filters with the cryptophyte were frozen (−80 °C) and added phosphate buffer for extraction at 4 °C followed by absorbance measurement. The cryptophyte Rhodomonas salina was used as a model organism.•Simple method for extraction and quantification of phycoerythrin from cryptophytes.•Minimal usage of equipment and chemicals, and low labor costs.•Applicable for industrial and biological purposes.

KW - Labor cost

KW - Microalgae

KW - Phycobiliprotein

KW - Pigment

KW - Processing time

KW - Rhodomonas salina

U2 - 10.1016/j.mex.2017.06.002

DO - 10.1016/j.mex.2017.06.002

M3 - Journal article

SN - 2215-0161

VL - 4

SP - 209

EP - 213

JO - MethodsX

JF - MethodsX

IS - C

ER -

A simple and fast method for extraction and quantification of cryptophyte phycoerythrin

Abstract

Keywords

Link to document

Projects

COMA: COpepod egg Mass production in Aquaculture

Cite this