TY - JOUR
T1 - A serosurvey examining exposure to Borrelia burgdorferi sensu lato and tick-borne encephalitis virus in Danish blood donors, August 2022
AU - Hansen, Mette Frimodt
AU - Gynthersen, Rosa Maja Møhring
AU - Ocias, Lukas Frans
AU - Sørensen, Camilla Adler
AU - Jensen, Bitten Aagaard
AU - Erikstrup, Christian
AU - Holm, Dorte Kinggaard
AU - Sækmose, Susanne Gjørup
AU - Harritshøj, Lene Holm
AU - Kolstad, Linda
AU - Hoffman, Tove
AU - Lundkvist, Åke
AU - Mens, Helene
AU - Lebech, Anne Mette
AU - Krogfelt, Karen Angeliki
N1 - Funding Information:
The project was partially supported by the InterReg North Sea Region programme, NorthTick, Grant no. J-No.: 38-2-7-19.
PY - 2024/9
Y1 - 2024/9
N2 - Objectives: Borrelia burgdorferi sensu lato (Bbsl) and tick-borne encephalitis virus (TBEV) are tick-borne pathogens. This study aimed to investigate the seroprevalence of these pathogens in Danish blood donors. Methods: A total of 1000 plasma samples equally distributed (n = 200) from all five Danish regions were analyzed. Commercially available enzyme-linked immunosorbent assays were used to screen the samples for immunoglobulin G antibodies against Bbsl and TBEV. The samples positive for antibodies against TBEV were further examined with a commercially available enzyme-linked immunosorbent assay and a Luminex-based TBEV suspension multiplex immunoassay for specific antibodies against non-structural protein 1 (NS1) antigen suggestive of previous infection. Results: A total of 62 samples tested positive for immunoglobulin G antibodies against Bbsl. A total of 40 samples were positive or borderline for antibodies against TBEV, indicating potential infection or vaccination. Of these, one had antibodies against NS1, indicating past infection. The seroprevalence of Bbsl was 6.2% (95% confidence interval 4.8-7.8), with equal seroprevalence in all five regions. The seroprevalence of TBEV was 0.1% (95% confidence interval 0.01-0.62%). Conclusions: The seroprevalence of Bbsl was similar throughout the country and corresponds well with previous studies. The seroprevalence of TBEV NS1 was low, which is in line with a low number of reported tick-borne encephalitis cases in Denmark. The NS1 positive sample was from the Capital Region, an endemic TBEV area.
AB - Objectives: Borrelia burgdorferi sensu lato (Bbsl) and tick-borne encephalitis virus (TBEV) are tick-borne pathogens. This study aimed to investigate the seroprevalence of these pathogens in Danish blood donors. Methods: A total of 1000 plasma samples equally distributed (n = 200) from all five Danish regions were analyzed. Commercially available enzyme-linked immunosorbent assays were used to screen the samples for immunoglobulin G antibodies against Bbsl and TBEV. The samples positive for antibodies against TBEV were further examined with a commercially available enzyme-linked immunosorbent assay and a Luminex-based TBEV suspension multiplex immunoassay for specific antibodies against non-structural protein 1 (NS1) antigen suggestive of previous infection. Results: A total of 62 samples tested positive for immunoglobulin G antibodies against Bbsl. A total of 40 samples were positive or borderline for antibodies against TBEV, indicating potential infection or vaccination. Of these, one had antibodies against NS1, indicating past infection. The seroprevalence of Bbsl was 6.2% (95% confidence interval 4.8-7.8), with equal seroprevalence in all five regions. The seroprevalence of TBEV was 0.1% (95% confidence interval 0.01-0.62%). Conclusions: The seroprevalence of Bbsl was similar throughout the country and corresponds well with previous studies. The seroprevalence of TBEV NS1 was low, which is in line with a low number of reported tick-borne encephalitis cases in Denmark. The NS1 positive sample was from the Capital Region, an endemic TBEV area.
KW - Borrelia burgdorferi sensu lato
KW - Lyme borreliosis
KW - Seroprevalence
KW - Serosurvey
KW - TBE
KW - Tick borne pathogens
KW - Tick-borne encephalitis virus
KW - Borrelia burgdorferi sensu lato
KW - Lyme borreliosis
KW - Seroprevalence
KW - Serosurvey
KW - TBE
KW - Tick borne pathogens
KW - Tick-borne encephalitis virus
U2 - 10.1016/j.ijregi.2024.100414
DO - 10.1016/j.ijregi.2024.100414
M3 - Journal article
AN - SCOPUS:85201915601
SN - 2772-7076
VL - 12
JO - IJID Regions
JF - IJID Regions
M1 - 100414
ER -