A comprehensive and precise quantification of the calanoid copepod Acartia tonsa (Dana) for intensive live feed cultures using an automated ZooImage system

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The copepod Acartia tonsa (Dana) is a promising live feed species for marine larviculture. A considerable progress has been made on the culturing technique for mass production, yet the application of automatic systems for quantification of A. tonsa development stages and biomass has largely been ignored. In this study, we propose a novel method for highly precise classification of development stages and biomass of A. tonsa, in intensive live feed cultures, using an automated ZooImage system, a freeware image analysis. We successfully created a training set of 13 categories, including 7 copepod and 6 non-copepod (debris) groups. ZooImage used this training set for automatic discrimination through a random forest algorithm with the general accuracy of 92.8%. The ZooImage showed no significant difference in classifying solitary eggs, or mixed nauplii stages and copepodites compared to personal microscope observation. Furthermore, ZooImage was also adapted for automatic estimation of A. tonsa biomass. This is the first study that has successfully applied ZooImage software which enables fast and reliable quantification of the development stages and the biomass of A. tonsa. As a result, relevant culturing parameters in intensive A. tonsa culture such as hatching success, mortality rate, development rate, and reproduction output of A. tonsa can be calculated automatically. Moreover, ZooImage is set up with an inexpensive desktop scanner and computer that can easily be applied in aquaculture hatcheries
Original languageEnglish
Article number422-423
JournalAquaculture
Volume422-423
Pages (from-to)225-231
Number of pages7
ISSN0044-8486
DOIs
Publication statusPublished - 20 Feb 2014

Keywords

  • Copepod culture
  • Acartia tonsa
  • Quantification
  • ZooImage

Cite this

@article{3f935d18012e4173ad5700a010e2b00c,
title = "A comprehensive and precise quantification of the calanoid copepod Acartia tonsa (Dana) for intensive live feed cultures using an automated ZooImage system",
abstract = "The copepod Acartia tonsa (Dana) is a promising live feed species for marine larviculture. A considerable progress has been made on the culturing technique for mass production, yet the application of automatic systems for quantification of A. tonsa development stages and biomass has largely been ignored. In this study, we propose a novel method for highly precise classification of development stages and biomass of A. tonsa, in intensive live feed cultures, using an automated ZooImage system, a freeware image analysis. We successfully created a training set of 13 categories, including 7 copepod and 6 non-copepod (debris) groups. ZooImage used this training set for automatic discrimination through a random forest algorithm with the general accuracy of 92.8{\%}. The ZooImage showed no significant difference in classifying solitary eggs, or mixed nauplii stages and copepodites compared to personal microscope observation. Furthermore, ZooImage was also adapted for automatic estimation of A. tonsa biomass. This is the first study that has successfully applied ZooImage software which enables fast and reliable quantification of the development stages and the biomass of A. tonsa. As a result, relevant culturing parameters in intensive A. tonsa culture such as hatching success, mortality rate, development rate, and reproduction output of A. tonsa can be calculated automatically. Moreover, ZooImage is set up with an inexpensive desktop scanner and computer that can easily be applied in aquaculture hatcheries",
keywords = "Copepod culture, Acartia tonsa, Quantification, ZooImage, Copepod culture, Acartia tonsa, Quantification, ZooImage",
author = "Vu, {Minh Thi Thuy} and Jepsen, {Per Meyer} and Hansen, {Benni Winding}",
year = "2014",
month = "2",
day = "20",
doi = "10.1016/j.aquaculture.2013.12.002",
language = "English",
volume = "422-423",
pages = "225--231",
journal = "Aquaculture",
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A comprehensive and precise quantification of the calanoid copepod Acartia tonsa (Dana) for intensive live feed cultures using an automated ZooImage system. / Vu, Minh Thi Thuy; Jepsen, Per Meyer; Hansen, Benni Winding.

In: Aquaculture, Vol. 422-423, 422-423, 20.02.2014, p. 225-231.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - A comprehensive and precise quantification of the calanoid copepod Acartia tonsa (Dana) for intensive live feed cultures using an automated ZooImage system

AU - Vu, Minh Thi Thuy

AU - Jepsen, Per Meyer

AU - Hansen, Benni Winding

PY - 2014/2/20

Y1 - 2014/2/20

N2 - The copepod Acartia tonsa (Dana) is a promising live feed species for marine larviculture. A considerable progress has been made on the culturing technique for mass production, yet the application of automatic systems for quantification of A. tonsa development stages and biomass has largely been ignored. In this study, we propose a novel method for highly precise classification of development stages and biomass of A. tonsa, in intensive live feed cultures, using an automated ZooImage system, a freeware image analysis. We successfully created a training set of 13 categories, including 7 copepod and 6 non-copepod (debris) groups. ZooImage used this training set for automatic discrimination through a random forest algorithm with the general accuracy of 92.8%. The ZooImage showed no significant difference in classifying solitary eggs, or mixed nauplii stages and copepodites compared to personal microscope observation. Furthermore, ZooImage was also adapted for automatic estimation of A. tonsa biomass. This is the first study that has successfully applied ZooImage software which enables fast and reliable quantification of the development stages and the biomass of A. tonsa. As a result, relevant culturing parameters in intensive A. tonsa culture such as hatching success, mortality rate, development rate, and reproduction output of A. tonsa can be calculated automatically. Moreover, ZooImage is set up with an inexpensive desktop scanner and computer that can easily be applied in aquaculture hatcheries

AB - The copepod Acartia tonsa (Dana) is a promising live feed species for marine larviculture. A considerable progress has been made on the culturing technique for mass production, yet the application of automatic systems for quantification of A. tonsa development stages and biomass has largely been ignored. In this study, we propose a novel method for highly precise classification of development stages and biomass of A. tonsa, in intensive live feed cultures, using an automated ZooImage system, a freeware image analysis. We successfully created a training set of 13 categories, including 7 copepod and 6 non-copepod (debris) groups. ZooImage used this training set for automatic discrimination through a random forest algorithm with the general accuracy of 92.8%. The ZooImage showed no significant difference in classifying solitary eggs, or mixed nauplii stages and copepodites compared to personal microscope observation. Furthermore, ZooImage was also adapted for automatic estimation of A. tonsa biomass. This is the first study that has successfully applied ZooImage software which enables fast and reliable quantification of the development stages and the biomass of A. tonsa. As a result, relevant culturing parameters in intensive A. tonsa culture such as hatching success, mortality rate, development rate, and reproduction output of A. tonsa can be calculated automatically. Moreover, ZooImage is set up with an inexpensive desktop scanner and computer that can easily be applied in aquaculture hatcheries

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