1 kb of the lactase-phlorizin hydrolase promoter directs post-weaning decline and small intestinal-specific expression in transgenic mice

Jesper T. Troelsen*, Anja Mehlum, Jørgen Olsen, Nikolaj Spodsberg, Gert H. Hansen, Hans Prydz, Ove Norén, Hans Sjöström

*Corresponding author

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Adult-type hypolactasia is a genetic condition making approximately one half of the human population intolerant to milk because of abdominal symptoms. The cause is a post-weaning down-regulation of the intestinal-specific enzyme lactase-phlorizin hydrolase (LPH) reducing the intestinal capacity to hydrolyze lactose. We here demonstrate that the stretch -17 to -994 in the pig LPH-promoter carries cis-elements which direct a small intestinal-specific expression and a post-weaning decline of a linked rabbit β-globin gene. These data demonstrate that the post-weaning decline of LPH is mainly due to a transcriptional down-regulation.

Original languageEnglish
JournalFEBS Letters
Volume342
Issue number3
Pages (from-to)291-296
Number of pages6
ISSN0014-5793
DOIs
Publication statusPublished - 11 Apr 1994
Externally publishedYes

Keywords

  • Enterocyte
  • Hypolactasia
  • Lactase-phlorizin hydrolase
  • Lactose intolerance
  • Small intestine
  • Tissue specificity

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