This project investigates whether or not the presence of microplastics in the environment changes the effect, biotransformation and bioaccumulation of pyrene in the earthworm Eisenia fetida. Three experiments are performed, two of which act as pilot experiments for the last main experiment. The two pilot experiments determine E. fetida’s food requirements, so as not to involuntarily starve the test organism during the main experiment, and E. fetida’s tolerance of various pyrene concentrations, to serve as a comparison with later experimental results and an indicator of toxicity to the test organism. The main experiment determines the combined effects of pyrene and microplastic on E. fetida, followed by an analysis of pyrene concentrations in tissue samples of E. fetida from the main experiment using HPLC-MS. It is concluded that there is a positive interaction between bioaccumulation of pyrene in E. fetida and the presence of microplastics and that further investigation into the relationship between microplastics and environmental contaminants in both aquatic and terrestrial ecosystems is needed.
|Uddannelser||Basis - International Naturvidenskabelig Bacheloruddannelse, (Bachelor uddannelse) Bachelor|
|Udgivelsesdato||19 jan. 2016|
- Eisenia fetida