Cell growth and cell proliferation are two very important processes for multicellular organisms. In mammalian systems these processes are coordinated by a protein-complex mTOR, which is a central regulator of a complex signaling transduction pathway. TSC1 is an upstream protein of mTOR pathway that has a negative effect on the mTOR itself and, therefore, on the cell growth and cell proliferation. Recently it has become evident that resveratrol has an impact on the cell growth and the cell proliferation as well. In this report we have tried to find out: What is the influence of resveratrol treatment in combination with tsc1 knock out on the mammalian cell growth and cell proliferation? A series of experiments on TSC1+/+ and TSC1-/- mouse embryonic fibroblast cells was carried out. The results of our investigation show that resveratrol treatment inhibits the cell growth and proliferation for both TSC1+/+ and TSC1-/- cell lines in both Akt-dependent and independent way. However, in the TSC1-/- cells these two processes were relieved through the mTOR pathway. Besides that, another observation was made, showing the size of TSC1+/+ and TSC1-/- cells, which is controlled by the interaction between the cell growth and cell proliferation, were changing very differently in time- and concentration-dependent manner. The results of this investigation are not very accurate, but can provide an answer to the research question in general lines.
|Uddannelser||Basis - International Naturvidenskabelig Bacheloruddannelse, (Bachelor uddannelse) Basis|
|Udgivelsesdato||21 jun. 2007|
- Cell growth and cell proliferation