The Effect of Ntg2 on MMR in S. cerevisiae

Mie G Andersen & Ane N Ethelberg

Studenteropgave: Semesterprojekt

Abstrakt

This project has two aims. First it aims to investigate the role of Ntg2 in the mismatch repair system (MMR) in yeast cells. Ntg2 is an A/P-glycosylase which is part of the base excision repair (BER) system in S. cerevisiae. It has been found that Ntg2 binds MLH1 which is a major component of MMR in eukaryotes. It has also been shown that over expression of Ntg2 in WT yeast cells leads to higher mutation frequency in a canavanine resistance assay, as if the MMR system did not function properly Second, the project aims to examine the inserts of the plasmids used in an earlier work. This earlier study concluded that human EXO1 could not complement MMR in a ScEXO1 deficient yeast strain. By sequencing the inserts of the plasmids used in this earlier study, we wished to prove that the results of the complementation study was not due to mutations in the inserts of the plasmids used.

UddannelserMolekylærbiologi, (Bachelor/kandidatuddannelse) Bachelor el. kandidat
SprogEngelsk
Udgivelsesdato1 jan. 2006

Emneord

  • MMR
  • BER