The Eae2 region located on chromosome 15 in mice are known to influence the development of CIA. Experiments have showed that the Cia30 region in Eae2 have influence on the CIA development in mice from the B10.RIII strain which is susceptible to CIA. The Adamts12, which function still are unknown, are located in the Cia30 region. Adamts12 has not only been observed in elevated concentrations in arthritis patients compared to healthy individuals but have also been shown to bind and degrade COMP a matrix protein present in cartilage. In the light of these results the Adamts12 could be a potentially candidate gene for de development of arthritis. To examine if the Adamts12 has any influence on CIA development there was preformed sequencing and expression analyses on two mouse strains. The strains used were the susceptible strain B10.RIII and the congenic strain B10.RIIICia30 which have homozygous genes from the CIA resistant RIIIS/J strain in the Cia30 region. Neither the sequencing with the PCR technique or the expression analysis with real-time PCR method did succeed properly which suggest that the Adamts12 is expressed in very low concentrations.
|Uddannelser||Molekylærbiologi, (Bachelor/kandidatuddannelse) Bachelor el. kandidat|
|Udgivelsesdato||16 jan. 2008|