Resveratrol- and Rapamycin-induced Morphological Changes to Human Epithelial Colon Cancer DLD-1 Cells

Jon Ambæk Durhuus & Ping Fang

Studenteropgave: Semesterprojekt

Abstrakt

The prosperous polyphenol resveratrol have shown healthy benefits and anti-cancer properties, but the effect of resveratrol on cell morphology is less studied. The antisuppressant rapamycin, targets mTOR (the mammalian target of rapamycin), which exist in two complexes in mammals (mTORC1 and -2), and have in recent studies controversially showed that both complexes are inhibited by rapamycin. Resveratrol seems to somehow to interact with the mTOR and PI3K-Akt and the treatment of rapamycin plus resveratrol is therefore interesting to study. The PI3K-Akt pathway is known as an important role of cell growth and migration by regulating F-actin (filamentous actin) assembly. The morphological changes to the human epithelial colon cancer cell line, DLD-1, treated with 60 µM resveratrol and 0.5 µM rapamycin was scanned with atomic force microscopy (AFM). The cells were incubated for 48 and 72 hours, fixated and scanned in air, and hereafter size and height were measured. The results show that resveratrol enhance the area about 8 times, when compared to untreated and rapamycin-treated cells. Resveratrol plus rapamycin increase the area but less than the sole resveratrol treatment. Incubation of resveratrol and/or rapamycin decreases the height of the cells.

UddannelserMolekylærbiologi, (Bachelor/kandidatuddannelse) Bachelor el. kandidat
SprogEngelsk
Udgivelsesdato22 jan. 2008
VejledereOle Vang

Emneord

  • morphology
  • mTOR
  • AFM
  • Akt
  • resveratrol
  • rapamycin