Probiotic manipulation of the gut microbiota in relation to inheritance in mice models

Diana Worm Jensen, Kira Riber Mygind, Farah Naz Rasool, Yasemin Özen, Naba Al-Sari & Christian B. Rasmussen

Studenteropgave: Semesterprojekt

Abstrakt

The human gut microbiota (GM) has shown to be engaged with the host immune system, thus having an impact on a broad range of diseases. GM can be manipulated by probiotics and thus influence the balance of the immune system. Evidence suggests that the GM is inherited from mother to offspring. Change in GM by probiotics and the inheritance from mother to offspring are investigated in mice models in our project. Methods: A litter of 10 BALB/cJ dams were given either 1-4*109 CFU/day of probiotics or PBS on a cheerio throughout pregnancy until day 21 days after birth. Faecal samples were collected from mothers at day 21 and pups at day 27. The QIAamp Fast DNA stool mini kit was used to extract bacterial DNA. Using primer HDA1 and HDA2 a PCR amplification of region V3 and V4 of the 16S rDNA gene were performed. DGGE was conducted to reveal the bacterial diversity in the samples. Band intensities and the differences between the bands were tested for statistical significance in BioNumerics Version 7.5. Results: No significant relationship between the mothers and their pups in either group was found in the PCA analysis. In the discriminant analysis, distinction between microbiotas from mothers and pups was clear, but there were no distinctions between mothers given probiotics and mothers given PBS. However, we did find two bands that were group-specific within the group of pups, but this difference is too small to indicate any large biological relevance. Conclusion: Based on our results, the probiotics do not change the GM in the mother mice and therefore no inheritance was observed. However, further analysis of the two deviating bands could reveal if it were probiotics or an effect of the probiotics. This could possibly be an indication of inheritance.

UddannelserBasis - Naturvidenskabelig Bacheloruddannelse, (Bachelor uddannelse) Basis
SprogEngelsk
Udgivelsesdato17 jun. 2015
VejledereKenneth Klingenberg Barfod

Emneord

  • microbiota
  • BALB/cJ
  • mice models
  • inheritance
  • DGGE
  • probiotics