Oxidative stress' effect on replication

Marietta Bækdal Gugerel, Josephine Bjergbæk Olsson & Stefan Profft

Studenteropgave: Semesterprojekt

Abstrakt

E. coli bacteria have multiple defense mechanisms for protection the cells against oxidative stress, that they encounter in their natural environments. One of these defense mechanisms is the protein Dps, which can protect the bacteria against ROS. Dps has the ability to regulate the condensation of DNA and to regulate initiation of replication by binding to DnaA. The number of replication forks, and thereby the number of origins, has an influence on the speed of replication. We Roskilde University Molecular biology tried to test if increase in the number of replication forks lead to oxidative stress. Furthermore we tried to test if cells with ∆ Dps were more exposed to oxidative stress. All though many mechanisms influence the replication speed, we believe that insertion of ectopic origins and the protein Dps can have an impact of the total replication speed. Our experiment showed a difference in the doubling times of ~ 20 minutes between the wild-type and the strains with two origins.

UddannelserMolekylærbiologi, (Bachelor/kandidatuddannelse) Bachelor el. kandidat
SprogEngelsk
Udgivelsesdato21 dec. 2015
VejledereOle Skovgaard

Emneord

  • Oxidative stress
  • ectopic origin
  • Flow cytometry
  • P1vir transduction
  • FLP-FRT rekombination
  • Dps
  • Replication speed
  • Biology
  • Replication forks