Nanotoxicity of Copper Oxide and Gold during the developmental stages of Drosophila melanogaster

Malthe Bjerring Andresen, Theodora Chrysoulidou, Maxime Raphaël Valentin Garnier, Marie Anne Catherine Stephanie Guillaume, Joseph Giegerich & Valentin Fievet

Studenteropgave: Fagmodulprojekt

Abstrakt

Nanotechnology has seen major progress in recent years, leading to promising new approaches
to improve industrial production, food preservation and human health. However,
some nanoparticles have been proven to be toxic to the environment and humans.
In this context, laboratory trials were conducted to determine the toxicity of Gold and
copper nanoparticles on the model organism Drosophila melanogaster upon ingestion. D.
melanogaster has distinct developmental stages, is inexpensive to keep, has a short life cycle
and developmental pathway similarities with vertebrates, thus serving as an ideal in vivo
model to test toxicological effects of nanoparticles. The experiments screened for pupation
and eclosion rates and generation of Reactive Oxygen Species (ROS) in the midgut.
In vivo toxicity was observed for 80nm Gold and 50nm Copper oxide upon oral ingestion,
as damage accumulated in the midgut of D. melanogaster and production of ROS was observed.
Additionally, dose-dependent developmental delay was observed using 80nm Gold
and 50nm Copper Oxide, but despite 40nm Gold resulting in ROS generation, little to no
developmental delay was observed. The outcome of the experiment should be considered
inconclusive due to prevalent lack of concordance of the results, but they can serve as a
guide for studies in the future.

UddannelserBasis - International Naturvidenskabelig Bacheloruddannelse, (Bachelor uddannelse) Basis
SprogEngelsk
Udgivelsesdato16 dec. 2019
Antal sider46
VejledereMorten Erik Møller