MicroRNA-29a and the mitochondrial target proteins Aldh2/Ube2z

Panagiotis Praftsas & Atanas Dimchev Dilov

Studenteropgave: Basisprojekt


Diabetes type 2 being a growing problem in society. The research regarding this sickness is gradually going into depth. An association has been shown between dysfunctional mitochondria and diabetes and we support that potential of microRNAs to regulate mitochondrial proteins might be contributing factor. These non-coding RNA molecules, called miRNAs, seem to have a relation to many diseases like diabetes type 2 and they function as regulators of gene expression by binding to the 3’ UTR region of mRNAs and destabilizing them or inhibit their translation. A number of studies show that miRNAs play pivotal role in the etiology and pathogenesis of Diabetes mellitus and its complications. The aim of this project is to investigate the connection between the mitochondrial proteins Aldh2/Ube2z upon their down-regulation and over-expression of miRNA-29a and furthermore the possible connection with type 2 diabetes. Databases show that the proteins Aldh2/ Ube2z are predicted to bind in ‘3’UTR region of miR-29a. Trying to indentify whether down-regulation of Aldh2/Ube2z leads to over-expression of miR-29a luciferase gene reporter was used. Based on the results achieved, luciferase gene reporter showed lower activity which brought to the conclusion that translation was prevented, and a down-regulation of Aldh2/Ube2z and over-expression of miR-29a was established.

UddannelserBasis - Naturvidenskabelig Bacheloruddannelse, (Bachelor uddannelse) Bachelor
Udgivelsesdato13 jun. 2016
Antal sider25