The human intestine is colonized by trillions of microbial communities. These microorganisms, especially bacteria, are commonly referred to as the gut microbiota. Experimental evidence suggests that interactions between the gut microbiota and cells of the host influence metabolic diseases including diabetes. Several metagenomic studies have investigated the mechanisms involved in the microbial influence on disease. Some bacteria are able to produce the short chain fatty acid butyrate. It has been suggested that butyrate plays a vital role in the regulation of glucose-metabolism, thus butyrate-producing bacteria have been associated with diabetes. The aim of this study was to investigate the indicated correlation between butyrate-producing bacteria and type 2 diabetes. Our study was based on a metagenome-study involving 145 70-year-old European women. Six of these women were used in this study, three diagnosed with type 2 diabetes and three with a normal glucose tolerance. A reference-catalogue was designed based on 18 bacterial strains. The abundance of these 18 bacterial strains was investigated in faecal DNA from the six women using the program R2R. The results of this study did not indicate a clear correlation between butyrate-producing bacteria and type 2 diabetes, as we did not find a general difference in the abundance of butyrate-producing bacteria in type 2 diabetic and healthy women. However, we did find that the microbiota is very specific to each woman and that the diversity of bacteria varies independent of the women’s state of health.
|Uddannelser||Molekylærbiologi, (Bachelor/kandidatuddannelse) Bachelor el. kandidat|
|Udgivelsesdato||23 jun. 2015|