Localization of antifreeze proteins in the European eelpout, Zoarces viviparus, by fluorescence microscopy

Johannes Lørup Johnsen

Studenteropgave: Semesterprojekt

Abstrakt

The European eelpout, Zoarces viviparus, is known to produce type III antifreeze proteins (AFP) when seasonal changes supercools its surrounding environment. Previous studies have showed that these antifreeze proteins are present in the serum during the colds months, but never before have they been localized to specific organs or tissues. Tissue was embedded in paraffin and sectioned on a microtome to produce both HE and immunofluorescence stains. Antibodies against type III AFP were raised in rabbit, and anti-rabbit antibodies containing fluorophores were used to visualize protein localization. The tissue showed much autofluorescence, but the striated muscle taken from the tail section of the fish showed fluorescence specific to the fluorophore wavelength in the endomysium. Some tissue was lost due to bad paraffin infiltration, and the results are therefore left inconclusive. Future immunofluorescence studies done on Z. viviparus should be done using fluorophores that emit near the DAPI wavelength to avoid autofluorescence.

UddannelserMolekylærbiologi, (Bachelor/kandidatuddannelse) Bachelor el. kandidat
SprogEngelsk
Udgivelsesdato20 mar. 2011
VejledereHans Ramløv

Emneord

  • histology
  • eelpout