Investigation of the interaction between point mutated EIIANtr and SsrB using two hybrid system in Salmonella causing chronic infection

Kamila Kamuda, Thomas Andersen, Esra Çetin, Ӧzge Yetiş & Romain Khoury

Studenteropgave: Semesterprojekt

Abstrakt

In the chronic infections caused by Salmonella enterica Typhimurium; bacteria reside in places such as mesenteric lymph nodes, spleen or liver (Monack, Bouley & Falkow, 2004). Chronic carriers do not show the disease symptoms but can still transmit it to others by shedding in stools as a major mechanism in spreading the disease (Griffin & McSorley, 2011). Single point mutation in ptsN (ptsN-SNP) gene resulted in complete dominancy over the wild type version of the gene in S.Typhimurium during chronic infection of mice. It is believed that the protein encoded by ptsN (EIIANtr) is involved in transport of phosphoryl group in sugar metabolism, nitrogen metabolism, or as lately research suggests in potassium metabolism. Specifically for Salmonella, EIIANtr was found to interact with SsrB, what resulted in negative regulation of the gene expression from Salmonella pathogenicity island 2 (Choi et al., 2010). Using BACTH system we aimed to investigate interaction between EIIANtr-SNP and SsrB. However, our results do not indicate the interaction of SsrB with neither EIIANtr-SNP nor EIIANtr.

UddannelserBasis - International Naturvidenskabelig Bacheloruddannelse, (Bachelor uddannelse) Basis
SprogEngelsk
Udgivelsesdato16 jun. 2015
VejledereLotte Jelsbak

Emneord

  • Salmonella
  • EIIANtr
  • chronic infection
  • ssrB
  • BACTH
  • ptsN
  • two hybrid system
  • S.Typhimurium