Investigation of synergy mechanism between TrCel7A and TrCel12A

Zara Alam, Kamila Kamuda, Thomas Andersen, Josh Shailes & Nanna Rolsted

Studenteropgave: Semesterprojekt

Abstrakt

The purpose of this project was to examine if TrCel12A facilitates the potential of TrCel7A to hydrolyse cellulose by creating more attack points or more exit points. This synergy of the two cellulases was investigated with two different investigation approaches - a time-dependent biosensor measurement and a time-independent PAHBAH measurement. An increase in Vmax was observed for the treated samples compared to untreated samples. The Vmax values for; untreated, 1 hour pre-treated and ON pre-treated were 1.35*10-2 µM/s, 4.08*10-2 µM/s and 3.91*10-2 µM/s respectively. Additionally, increases in koff rate vales were shown for the treated samples compared to the untreated samples also showing a slight reduction in processivity for the pre-treated. The koff rates observed for; untreated, 1 hour and ON were 4.448*10-3 , 1.506*10-2 and 2.946*10-2 µM/s respectively. This suggests in line with other literature that TrCel12A cleaves proximally to amorphous regions creating more exit points and reducing amount of obstacle hindrance that TrCel7A would typically encounter.

UddannelserKemi, (Bachelor/kandidatuddannelse) Bachelor el. kandidat
SprogEngelsk
Udgivelsesdato25 dec. 2015
VejledereJeppe Kari

Emneord

  • synergy
  • TrCel7A
  • Cellulases
  • Cellulose
  • TrCel12A
  • Michaelis Menten
  • Processivity
  • mechanism
  • Biosensors