Interaction of Ferritin from Halophilic Bacteria with Iron

Peter Rejic Puhar & Alex Charles Sabin

Studenteropgave: Bachelorprojekt

Abstrakt

Ferritin is a protein found in nearly all cells and its function is to collect the toxic iron(II) freely floating inside the cells and oxidise it to iron(III) and storing it inside the cavity located in the center of the protein. While iron ions are natural targets of ferritin, it has also displayed the ability to perform the same reactions with other metal ions, most notably ions from the heavy metal group. This project and its experiments only focus on iron. The protein ferritin from the halophilic bacteria Natronolimnobius Innermongolicus was produced and used in doing basic kinetic measurements, as well as in preparing a special sample of crystallized ferritin loaded with iron for Mössbauer spectroscopy, which is conducted by prof. Mikkel Fougt Hansen at DTU. The results of the kinetic tests imply that changes in pH affect the iron uptake rate, while effects of NaCl and minimal availability of iron are negligible.

UddannelserBasis - International Naturvidenskabelig Bacheloruddannelse, (Bachelor uddannelse) Bachelor
SprogEngelsk
Udgivelsesdato28 maj 2018
Antal sider29
VejledereKornelius Zeth