Increasing Solubility of Dexamethasone by Complexation with Beta and Gamma Cyclodextrins

Karina Gaardahl & Bryan Tobias Houston

Studenteropgave: Bachelorprojekt


The solubility of dexamethasone (DMS) upon adding β- and/or γ- cyclodextrin (CD), was measured by the absorbance at 250 nm and was determined with HPLC. The concentrations of DMS were plotted against the amount of added cyclodextrin to yield a Phase-Solubility Diagram. Three sets of samples were prepared, one for each type of cyclodextrin individually, and one where both types were added. The first two were used to find the concentration of DMS in solution at different amounts of added CD, and yielded B-type Phase-Solubility Diagrams. The βCD complexes had a higher solubility seen by a concentration of dissolved DMS plateau value of 8.4 mM, whereas the γCD data had a plateau value of 7.4 mM. The third experiment was conducted to determine how the concentration of DMS was affected when adding multiple types of CD. The slope of the increase in solubility showed an additive effect, on solubility, from the combined cyclodextrins, however the results indicate that there is a synergistic effect on the maximum solubility of DMS.

UddannelserKemi, (Bachelor/kandidatuddannelse) BachelorMatematik, (Bachelor/kandidatuddannelse) BachelorMedicinalbiologi, (Bachelor/kandidatuddannelse) BachelorMolekylærbiologi, (Bachelor/kandidatuddannelse) Bachelor
Antal sider57
VejledereJens Christian Sidney Schönbeck